The objective of this study was to investigate the in vitro antioxidant activity of fermented milk of indigenous hill cattle (Himachali Pahari Cow) supplemented with various aqueous herbal extracts. The probiotic Lactobacillus rhamnosus GG (LGG) fermented milk supplemented with 1% aqueous herbal extracts of fruits of harad (Terminalia chebula), baheda (Terminalia bellerica), arjuna (Terminalia arjuna), and amla (Phyllanthus emblica) were evaluated for antioxidative activity. Fermented milk containing various aqueous herbal extracts,
The present study was conducted with the objective to enhance the Angiotensin converting enzyme (ACE, EC 3.4.15.1) inhibitory activity of indigenous cattle milk through supplementation with various herbal extracts. The fermented milk supplemented with aqueous extracts of fruits of harad (Terminalia chebula), baheda (Terminalia bellirica), amla (Phyllanthus emblica) and bark of arjuna (Terminalia arjuna) were evaluated for ACE inhibitory activity. Maximum (37.92±0.72%) ACE inhibitory activity was observed in pepsin-digest of fermented milk containing aqueous harad extract, whereas minimum (25.37±0.59%) ACE inhibitory activity was observed in fermented milk containing aqueous extract of amla. Undigested samples of fermented milk containing aqueous arjuna extract exhibited significantly higher ACE inhibitory activity (19.70±0.58%), followed by amla (17.00±0.70%), and baheda (16.59± 0.94%). Fermented milk containing aqueous harad extract had significantly lower value (14.97±0.62%). No significant difference was found between fermented milk containing aqueous extracts amla and extracts of baheda. Based on the results described above, we surmise that herbal supplemented fermented milk had better antihypertensive activity.
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