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Density and speed of sound of polyethylene glycols (polyethylene glycol 200; polyethylene glycol 400) in (0.000, 0.0024, 0.0032, and 0.0040) mol·Kg-1aqueous solutions of biotin at constant experimental pressure 0.1 MPa and temperature range (288.15 K, 298.15 K, 308.15 K and 318.15 K) have been obtained using Anton-Paar DSA 5000 M. These experimental data were used to calculate various acoustic and thermodynamic parameters such as intermolecular free length, acoustic impedance, adiabatic compressibility, Wada’s constant, Rao’s Constant and Vander Waal’s constant which gave the insight of the intermolecular reactions inside the ternary mixture of vitamin B7(Biotin) and polyethylene glycols (PEG 200/PEG 400).