This investigation aimed to assess the microbial quality of drinking water produced by drinking water filtration dispenser toll machines (DFTMs) to assess the health risk of DFTM water. A total of 210 samples were randomly collected of 70 DFTMs within a radial distance of 500 metres around Mahasarakham University. The prevalence of Escherichia coli and Staphylococcus aureus in the DFTM water was 54.17% and 16.67% with an average concentration of 1.04 log cfu/ml and 0.26 log cfu/ml, respectively. The risk calculation (using the @Risk program based on drinking water consumption of 2 L/day) indicated that the probability of exposure was 1.67 E-01, while the probability of illness due to E. coli and S. aureus was 2.08 E-03 and 1.58 E-05 and the risk of illness was 1.13 E-03 and 2.64 E-06, respectively. The sanitation status and drinking water quality related to health would be influenced by other pathogens due to the poor hygienic conditions of DFTMs and improper environments.