Summary:In this research, seroprevalence of BTV serotypes (BTV-4, BTV-9, BTV-16), and occurrence of Akabane and Ephemeral fever viruses were investigated in 557 cattle sera collected from 21 locations in European Turkey (Thrace). Virus neutralization test was used to discriminate antibody response against BTV serotypes while commercial blocking-ELISA systems were used to detect antibodies for Akabane and ephemeral fever viruses. The overall seroprevalence of BTV was recorded as 73.54%, comprising individual percentages for BTV-4, BTV-9 and BTV-16 were 69.04%, 71.38% and 80.2%, respectively. The seroprevalence of Ephemeral fever virus infection in Thrace varied between 2.5 and 15.3% on the province basis, whilst antibody percentage for Akabane virus was found as low as 0.14% in the study.Key words: Akabane, bluetongue, ephemeral fever, seroprevalence. Trakya bölgesinde sığırlarda sivrisineklerle nakledilen enfeksiyonların seroprevalansıÖzet: Trakya bölgesinde, 2003 yılında 5 ile bağlı 21 ilçe/köyden toplam 557 sığır, Mavidil, Akabane ve Ephemeral Fever enfeksiyonlarının seroprevalanslarının tespiti amacıyla random sampling metoduyla örneklendi. Serum örnekleri Mavidil virusunun 3 farklı serotipine (BTV-serotip 4, BTV-serotip 9 ve BTV-serotip 16) karşı virus nötralizasyon testine tabi tutuldu. Aynı serum örneklerine Akabane ve Ephemeral Fever virus antikorlarının tespiti için blocking ELISA testi uygulandı. Trakya bölgesinde mavi dil enfeksiyonunun seroprevalansı %73.54 olarak tespit edilirken, BTV serotip-4 %69.04, BTV serotip-9 %71.38, BTV serotip-16 ise %80.2 olarak saptandı. Akabane enfeksiyonunun seroprevalansı Trakya bölgesinde %0.14 olarak belirlenirken, Ephemeral fever enfeksiyonunun seroprevalansı örneklenen iller bazında %2.5-15.3 olarak tespit edildi.
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