There is little information on the ecology and geographic distribution of the rare species Vampyrum spectrum due to its low abundance and the paucity of records in Colombia. In a field trip to the Reserva Natural Bosque de Yotoco, Valle del Cauca, we captured a female Vampyrum spectrum in a mist net. It constitutes the first verified record of the species in Valle del Cauca, thus updating the geographic distribution of the species in the country, filling the information gap on the species for this zone and highlighting the importance of the Yotoco Forests as an area of interest for the conservation of the biological diversity of the region.