Objective To describe the results of treatment with CO2 laser for Bartholin gland cysts.Methods Thirty-one women with Bartholin gland cysts were treated with CO2 laser at an outpatient´s setting. Skin incision was performed with focused laser beam, the capsule was opened to drain mucoid content, followed by internal vaporization of impaired capsule.Results There were no complications. Five patients had recurrence of the cyst and were submitted to a second and successful session.Conclusion CO2 laser surgery was effective to treat Bartholin gland cysts with minimal or no complications, and can be performed at an outpatient´s setting.