We used the recent phylogenomic tree from Kolmann et al., (2021) to generate a phylogenetic framework to account for shared ancestry among species. Kolmann et al., (2021) used exon capture to generate a phylogeny of serrasalmids from 951 exons. The taxon sampling covers 63 of the currently recognized 100 serrasalmid species with representatives from all recognized serrasalmid genera. The exon probe set they used contains several exons that are legacy markers traditionally used in resolving fish phylogenetics (Arcila et al., 2017). To increase taxon sampling, we utilized six legacy markers sequenced by Kolmann et al., (2021) (COI, ENC1, FICD, IRBP, SREB2, and VCPIP) that have also been used to resolve fish phylogenetic relationships.We obtained additional sequences from GenBank for COI and SREB2 that have previously been used in resolving serrasalmid relationships (Thompson et al., 2014;Machado et al., 2018).Additionally, we added a marker not included in the exon dataset of Kolmann et al., (2021), PTR, which had previously been used for serrasalmid phylogenetics (Thompson et al., 2014). Two species, Utiaritichthys sennaebragai (SRX7670167) and Serrasalmus hollandi (SRX7670146), only have sequence data on the NCBI Sequence Read Archive from a recent ultraconserved elements (UCEs) study of serrasalmids (Mateussi et al., 2020). To add these species into our