This study was designed to determine the effect of phytase extracted from Aspergillus niger (Natuphos ® E) on growth performance, bone mineralization, phosphorous excretion, and meat quality parameters in broilers fed available phosphorous (aP)-deficient diet. In total, 810 one-day-old Indian River broilers were randomly allotted into one of three dietary treatments, with six replicates per treatment. The three dietary treatments were 1) control group (CON: basal diet with sufficient aP), 2) low phytase (LPY: available phosphorus-deficient diet supplemented with 0.01% phytase), and 3) high phytase (HPY: available phosphorus-deficient diet supplemented with 0.02% phytase). Average daily gain and, feed intake, and feed conversion ratio were measured for 35 days. Excreta were collected from each pen on day 35. One broiler from each cage was euthanized to collect visceral organs and tibia samples. Broiler chickens fed LPY and HPY showed improved (P＜0.05) growth performance compared to broilers fed CON on day 35. The tibia length of HPY-fed broilers was more than those of broilers fed other diets on day 35 (P＜0.05). However, tibia calcium and phosphorous contents in LPY-fed broilers was higher (P＜0.05) than in CON and HPYfed broilers. Tibia length and calcium and phosphorous content showed a positive correlation (P＜0.05) with the weight gain of broilers on day 35. Phosphorous level in the excreta of LPY-and HPY-fed broilers was lesser than those of CON broilers on day 35 (P＜0.05). Furthermore, HPY-fed broilers showed lower (P＜0.05) phosphorous content in the excreta than LPY-fed broilers. LPY-and HPY-fed broilers showed higher (P＜0.05) liver weight than the CON broilers. In conclusion, broilers fed aP-deficient diet supplemented with phytase from Aspergillus excreted less phosphorus, which enhanced growth performance and tibia development from time of hatching to day 35 posthatching.