Education evaluation should be based on students' viewpoints and interest. Based on that, the research was aimed to determine the importance of purpose oriented study and ability to show empathy to the school wellbeing. The proposed hypothesis was there is positive correlation between purpose-oriented study and ability to show empathy to the school well-being. The study involved 123 senior high school students based on simple random sampling method. Data were collected using the School Well-being Scale (20 items, α = .81), the Purpose-Oriented Study Scale (20 items; α = .77) and the Empathy Scale (24 items; α = .79). Regression analysis was used to test the hypothesis. The result shows that there is a significant and positive correlation between purpose-oriented study and ability to show empathy to the school well-being (r = .364; p < .001). It emphasizes the importance of using tolerated interactional teaching-learning process so that the students will be able to obtain learning meanings that are attached to the school environment.Keywords: school well-being, purpose oriented study, empathy, high school students AbstrakPenelitian ini dilakukan dengan tujuan untuk mengetahui keterkaitan dan peran orientasi belajar mencari makna dan kemampuan empati terhadap kesejahteraan sekolah. Urgensi yang menjadi landasan adalah perlunya peningkatan evaluasi pendidikan yang berdasar pada sudut pandang dan kepentingan siswa. Selain untuk mendapatkan deskripsi, penelitian ini dikembangkan dengan hipotesis bahwa terdapat korelasi positif yang signifikan antara orientasi belajar mencari makna dan kemampuan empati dengan kesejahteraan sekolah. Penelitian melibatkan 123 siswa Sekolah Menengah Atas, dengan teknik pengambilan sampel simple random sampling. Pengumpulan data dilakukan dengan menggunakan Skala Kesejahteraan Sekolah (20 aitem; α = 0,81), Skala Orientasi Belajar Mencari Makna (20 aitem;α = 0,77) dan Skala Empati (24 aitem; α = 0,79). Hasil analisis data dengan analisis regresi menunjukkan terdapat hubungan positif yang signifikan antara kesejahteraan sekolah dengan orientasi belajar mencari makna dan kemampuan empati (r = 0,364; p < 0,001). Hipotesis yang terbukti dapat menjadi dasar perlunya mengedepankan pembelajaran interaksional yang penuh toleransi dan memungkinkan siswa mendapatkan arti pembelajaran yang menyatu dengan lingkungan sekolah.Kata kunci: kesejahteraan sekolah, orientasi belajar mencari makna, kemampuan empati, siswa sekolah menengah atas PENDAHULUANPeningkatan mutu sekolah sebagai lembaga pendidikan merupakan masalah yang masih menjadi fokus utama di Indonesia, dari masalah infrastruktur bangunan yang jauh dari layak, sarana prasarana, mutu guru sebagai pengajar, maupun metode pembelajaran yang terus berusaha dikembangkan. Tujuan utama tentunya diarahkan pada keberhasilan dan efektifitas proses pendidikan. Muara dari tercapainya tujuan pendidikan tersebut tentunya bisa dilihat dari siswa yang menjadi subjek pendidikan.Perkembangan sosial siswa ditentukan oleh sekolah, sebagai salah satu faktor p...

Based on the person-environment congruence theory which argues that better fit with individual's environment will lead to more positive outcomes, we examined the relationships between the fit between individuals and organization (i.e., academic fit), the fit between individuals and their parents (i.e., adolescent-parent career congruence), and career exploration in Indonesian university students. We surveyed 125 students (53.6% girls, mean age = 18.96 years) majoring in Fisheries and Marine Science from a state university in Semarang, Central Java, using measures of academic fit, adolescent-parent career congruence, and career exploration. We examined whether adolescent-parent career congruence mediated the relationship between academic fit and career exploration. Structural equation modelling demonstrated that adolescent-parent career congruence partially mediated the relationship between academic fit and career exploration, suggesting that fitting with academic situation affects career exploration behavior by directly motivating students to engage in career exploration activities, and also by facilitating congruence with parents regarding career matter, that in turn, influences involvement in career exploration behavior. Findings of this study showed the vital roles for academic fit and adolescent-parent career congruence in students' career exploration behavior.

This study aims to explore the psychological well-being of individuals who were blind in early adulthood (late blind), particularly to understand the changes and the antecedents of their psychological well-being. Two adults, aged 20-40 years, who were blind in early adulthood, had participated in this study. This is a qualitative phenomenological study. Data were analyzed using the interpretative phenomenological analysis (IPA). The results showed that late blind brought several impacts on subjects. Both subjects identified a decreased communication skill, a reduced movement ability, and negative emotional response in the beginning of blindness as the antecedents of their psychological well-being. The increased of psychological well-being was obtained due to social support -in terms of emotional, informational, and instrumental support-from family, partner, friends, and community. Although both subjects applied different coping strategies, they both reported an increased psychological well-being after they reached self-acceptance dimension. This study revealed selfacceptance as initial dimension that helped subjects to reach other dimensions of psychological well-being: life goals, independence, environment mastery, personal growth, and positive relationships with others.Keywords: psychological well-being, blind, late blind, interpretative phenomenological analysis (IPA) AbstrakPenelitian ini bertujuan mengeksplorasi kesejahteraan psikologis pada individu yang menjadi tunanetra di usia dewasa awal, dan secara khusus berupaya memahami perubahan dan anteseden dari kesejahteraan psikologis mereka. Subjek penelitian adalah dua orang berusia 20-40 tahun yangmengalami kebutaan di usia dewasa awal. Penelitian ini adalah penelitian kualitatif yang menggunakan pendekatan fenomenologi. Data yang diperoleh dianalisis menggunakan metode interpretative phenomenological analysis (IPA). Kesejahteraan psikologis semua subjek mengalami perubahan akibat kebutaan yang dialaminya ketika usia dewasa awal. Di awal munculnya gejala, menurunnya kemampuan berkomunikasi, penurunan kemampuan mobilisasi, dan respon emosi negatif diungkap semua subjek sebagai anteseden menurunnya kesejahteraan psikologis mereka. Sedangkan peningkatan kesejahteraan psikologisdisebabkan oleh adanya dukungan sosial berupa dukungan emosi, informasi, dan instrumental yang bersumber dari keluarga, pasangan, teman, dan kelompok. Meskipun keduanya memiliki strategi coping yang berbeda, namun peningkatan kesejahteraan psikologis dapat dirasakan keduanya, setelah dimensi penerimaan diri berhasil diraihnya. Yang menarik adalah terungkapnya dimensi penerimaan diri sebagai dimensi awal yang membantu subjek dalam mencapai dimensi-dimensi kesejahteraan psikologis yang lain, yaitu tujuan hidup, kemandirian, penguasaan lingkungan, pertumbuhan personal, dan hubungan positif dengan orang lain.

In conjunction with other health promotion strategies, vaccination of coronavirus disease 2019 (COVID-19) is a strategy to alleviate the burden of infection. The aim of this study was to determine the differences in antibody response strength between individuals who received COVID-19 vaccination and those who had a natural infection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). A cross-sectional study was conducted among post-natural confirmed COVID-19 infection and immunized people in Bali, Indonesia. The vaccination was using Sinovac-CoronaVac with two-weeks interval between the two vaccine doses. To measure the level of anti-Spike receptor binding domain (SRBD) of SARS-CoV-2 antibody, we used Roche electro-chemiluminescence immunoassay (ECLIA) platform. Blood samples were obtained before and 28 days after first immunization in the vaccinated group, as well as two weeks after hospital discharge in the confirmed COVID-19 patients based on real-time reverse transcription polymerase chain reaction (RT-PCR). A total of 58 confirmed COVID-19 patients and 60 vaccinated individuals were included. On the 28th day after the initial vaccination, the seroconversion rate among vaccinated individuals was 91.67%. The mean titer of anti-SRBD SARS-CoV-2 antibody among vaccinated participants was 63.62±82.57 IU/mL (ranged between 0 IU/mL and 250 IU/mL). The mean titer among naturally infected group was 188.47±94.57 IU/mL (ranged between 4.25 IU/mL to 250 IU/mL) regardless the severity of COVID-19. Our data suggested that the titer of anti-SRBD SARS-CoV-2 antibody was significantly higher in naturally infected individuals compared to those who received COVID-19 vaccination (p<0.001). These data suggest that not all individuals vaccinated with Sinovac COVID-19 had protective level of anti-SRBD SARS-CoV-2 antibody and booster dose of heterologous vaccine maybe required.

In the previous study, we constructed an expression vector carrying the anti-EGFRvIII scFv antibody gene with VH-linker-VL orientation. The proteins were successfully produced in the periplasmic space of Escherichia coli. In this study, we substituted the inserted DNA with VL-linker-VH orientation of the anti-EGFRvIII scFv gene and analyzed its expression in E. coli. The DNA fragment was amplified from its cloning vector (pTz-rscFv), subsequently cloned into a previous expression vector containing the pelB signal sequence and his-tag, and then transformed into E. coli TOP10. The recombinant plasmids were characterized by restriction, PCR, and DNA sequencing analyses. The new anti-EGFRvIII scFv antibody proteins have been successfully expressed in the periplasmic compartment of E. coli Nico21(DE3) using 0.1 mM final concentration of IPTG induction. Total proteins, soluble periplasmic and cytoplasmic proteins, solubilized inclusion bodies, and extracellular proteins were analyzed by SDS-PAGE and Western Blot analyses. The results showed that soluble scFv proteins were found in all fractions except from the cytoplasmic space.