IVPure-transvaginal natural orifice transluminal endoscopic surgery (NOTES) ovariohysterectomy in bitches: a preliminary feasibility study

No abstract

PURPOSE:To evaluate the female sterilization by occlusion of the ovarian blood flow, using the rat as experimental model. METHODS:Fifty-five females rats were divided into four groups: I (n=10), bilateral ovariectomy, euthanized at 60 or 90 days; II (n=5), opening the abdominal cavity, euthanized at 90 days; III (n=20), bilateral occlusion of the ovarian blood supply using titanium clips, euthanized at 60 or 90 days; and IV (n=20), bilateral occlusion of the ovarian blood supply using nylon thread, euthanized at 60 or 90 days. The estrous cycle was monitored by vaginal cytology. After euthanasia, the reproductive tissues were evaluated histologically. RESULTS:Ovarian atresia was identified macroscopically at 60 days after surgery in the rats in groups III and IV; however, most of the rats in group III maintained cyclicity. Histology of the tissues from group IV revealed that the ovarian tissue was replaced by dense fibrous connective tissue that was slightly vascularized and that intact follicles were absent by 90 days. CONCLUSION:Ovarian blood vessels occluded caused ischemia, leading to progressive tissue necrosis, and bilateral occlusion using a nylon ligature is a viable method for surgical sterilization.

The occlusion of the inferior lacrimal punctum limits tear drainage and thus keeps it over the ocular surface, prolongs lubrication and minimizes the need of ocular lubricants. The aim of this study was to evaluate the effectiveness of n-butyl-cyanoacrylate and 2-octyl-cyanoacrylate adhesives in the occlusion of the inferior lacrimal punctum and canaliculi of dogs and their clinicalrepercussions over 180 days. Eleven dogs, were divided into two groups: occlusion with n-butyl-cyanoacrylate (GB, n=6) and with 2-octyl-cyanoacrylate (GO, n=5). Tear production, blepharospasm, ocular discharge, epiphora and conjunctival hyperemia were analyzed before the procedure and on days (T) 3,7,15, 30, 45, 60, 90, 120, 150 and 180 RESUMO A oclusão do ponto lacrimal inferior limita a drenagem da lágrima, mantendo-a sobre a superfície ocular, prolonga a lubrifi cação e minimiza a posologia de lubrifi cantes. Objetivou-se, com este estudo, avaliar a efetividade dos adesivos n-butil-cianoacrilato e 2-octil-cianocacrilato, na oclusão do ponto e canalículo lacrimais inferiores de cães e suas repercussões clínicas durante 180 dias. Para tanto, utilizaramse 11 cães distribuídos em dois grupos: oclusão com n-butilcianoacrilato (GB, n=6) e com 2-octil-cianoacrilato (GO; n=5).A produção lacrimal foi avaliada previamente ao procedimento e, posteriormente, aos 3, 7, 15, 30, 45, 60, 90, 120, 150