Objective: The decrement of 25-hydroxy-vitamin D [25-ΟΗ-D] concentrations has been increase the severity of cardiovascular disease especially in diabetic patients. The aim of the present study was to assess the effect of atorvastatin on 25-OH-D concentration in diabetic patients with hypercholesterolemia. Methods and patients: Forty diabetic patients with hypercholesterolemia were participated in this work. For comparison, thirty healthy subjects were inserted in the present study. Serum vitamin D levels and lipid profile (total cholesterol, triglyceride, HDL, LDL and VLDL) were measured. Results: Vitamin D levels significantly decrease (p˂0.05) in diabetic patients group (22.4±5.1 ng/ml) when compared to healthy group (32.44±4.12 ng/ml) .After 8 weeks from taking atorvastatin (20 mg/day) the mean of serum vitamin D were a slightly increase(26.4± 5.6) but not significant. Total cholesterol significantly decreases after therapy. Conclusion: Vitamin D levels significantly decrease in diabetic patients when compared to healthy subjects. There was no significant difference in vitamin D patients before and after lipid lowering therapy.