Rumination behavior in cattle is important for health and reproduction management of herd. The aim of presented paper was to evaluate change in rumination behavior in dairy cattle after changing total mixed ration (TMR) composition. Twenty-eight multiparous Red Holstein dairy cows were observed during rumination in first month after calving and in fourth month after calving. Cows were fed different total mixed ratio at beginning of lactation in first month after calving (TMR1) and in mid of lactation period in fourth month after calving (TMR2). TMR2 has higher content of roughage. Length of the single rumination period and number of jaw movements during rumination one bolus were recorded. Frequency of jaw movement per minute was calculated from recorded rumination characteristics. Rumination of one bolus TMR2 was longer (TMR1 = 53.97 ±8.241 sec, TMR2 = 57.57 ±6.290 sec) and cow perform more jaw movements (TMR1 = 61.0 ±10.674, TMR2 = 65.99 ±9.682) than ruminating bolus TMR1. Difference in duration of rumination one bolus as well as number of jaw movement was significant (P < 0.001). Frequency of jaw movement was not significantly affected (P > 0.05) and seem to be intra-individual stable. Rumination evaluated by correlation of rumination characteristics for TMR1 and TMR2 was intra-individual stable despite of changes in times of jaw movement and duration of one bolus rumination.