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Chemical characterization of complex mixtures by Nuclear Magnetic Resonance (NMR) spectroscopy is challenging due to a high degree of spectral overlap and inherently low sensitivity. Therefore, NMR experiments that reduce overlap and increase signal intensity hold immense potential for the analysis of mixtures such as biological and environmental media. Here, we introduce a 13 C version of DREAMTIME (Designed Refocused Excitation And Mixing for Targets In Vivo and Mixture Elucidation) NMR, which, when analyzing 13 C-enriched materials, allows the user to selectively detect only the compound(s) of interest and remove all other peaks in a 13 C spectrum. Selected peaks can additionally be "focused" into sharp "spikes" to increase sensitivity. 13 C-DREAMTIME is first demonstrated at high field strength (500 MHz) with simultaneous selection of eight amino acids in a 13 C-enriched cell free amino acid mixture and of six metabolites in an extract of 13 C-enriched green algae and demonstrated at low field strength (80 MHz) with a standard solution of 13 C-D-glucose and 13 C-L-phenylalanine. 13 C-DREAMTIME is then applied at high-field to analyze metabolic changes in 13 C-enrichedDaphnia magna after exposure to polystyrene "microplastics," as well as at low-field to track fermentation of 13 C-D-glucose using wine yeast. Ultimately, 13 C-DREAMTIME reduces spectral overlap as only selected compounds are recorded, resulting in the detection of analyte peaks that may otherwise not have been discernable. In combination with focusing, up to a 6-fold increase in signal intensity can be obtained for a given peak. 13 C-DREAMTIME is a promising experiment type for future reaction monitoring and for tracking metabolic processes with 13 C-enriched compounds.