The health of the honey bee Apis mellifera is challenged by introduced parasites that interact with its inherent pathogens and cause elevated rates of colony losses. To elucidate co‐occurrence, population dynamics, and synergistic interactions of honey bee pathogens, we established an array of diagnostic assays for a high‐throughput qPCR platform. Assuming that interaction of pathogens requires co‐occurrence within the same individual, single worker bees were analyzed instead of collective samples. Eleven viruses, four parasites, and three pathogenic bacteria were quantified in more than one thousand single bees sampled from sixteen disease‐free apiaries in Southwest Germany. The most abundant viruses were black queen cell virus (84%), Lake Sinai virus 1 (42%), and deformed wing virus B (35%). Forager bees from asymptomatic colonies were infected with two different viruses in average, and simultaneous infection with four to six viruses was common (14%). Also, the intestinal parasites Nosema ceranae (96%) and Crithidia mellificae/Lotmaria passim (52%) occurred very frequently. These results indicate that low‐level infections in honey bees are more common than previously assumed. All viruses showed seasonal variation, while N. ceranae did not. The foulbrood bacteria Paenibacillus larvae and Melissococcus plutonius were regionally distributed. Spearman's correlations and multiple regression analysis indicated possible synergistic interactions between the common pathogens, particularly for black queen cell virus. Beyond its suitability for further studies on honeybees, this targeted approach may be, due to its precision, capacity, and flexibility, a viable alternative to more expensive, sequencing‐based approaches in nonmodel systems.

Unravelling the taxonomy and distribution of two problematic small mammal genera in the Karoo biomeMain et al. Table S1: Location details for specimens sampled during this study. M. namaquensis Latitude: degrees South Longitude: degrees East Locations on map Marker RR1 −30.50666 21.34012 11 cytochrome b RR2 −30.50666 21.34012 11 cytochrome b RR3 −30.50666 21.34012 11 cytochrome b RR4 −30.50666 21.34012 11 cytochrome b RR5 −30.48179 21.35898 12 cytochrome b RR6 −30.48179 21.35898 12 cytochrome b RR7 −30.48179 21.35898 12 cytochrome b RR9 −30.48179 21.35898 12 cytochrome b RR10 −30.46297

Sub-Saharan Africa bears the highest disease burden in human and economic costs, but commands less than one percent of global health expenditure (Aikins et al., 2010).

Waste pickers are widely acknowledged as an integral part of the formal and informal economy, diverting waste into the secondary resource economy through urban mining. Urban mining in itself is considered to be a source of livelihoods. We investigated the livelihoods of e-waste pickers through 110 surveys in Cape Town, South Africa. Waste pickers often indicated that they were engaged in the sector not by choice but by necessity, expressing that earning money is the only enjoyable aspect of their job. The results from the study substantiate that it is unlikely that waste pickers could survive on e-waste picking alone as 83.3% of reported incomes were below minimum wage, with 22.9% below the food poverty line. Thus, the majority of waste pickers collected a wide array of recyclables. We also found that the waste pickers in Cape Town engage in multiple e-waste related activities, including collection, dismantling and processing to a lesser extent. They work long hours in arduous working conditions which present multiple hazards for their health and safety. Ultimately, e-waste pickers’ incomes cannot be considered commensurate with the nature of the work. Further, e-waste picking cannot be regarded to significantly contribute to livelihoods, but is rather a survivalist strategy. The survivalist nature of the work does not allow for waste pickers to move upwards in the waste value chain and benefit from greater income opportunities. Furthermore, their lack of skills prohibits waste pickers’ transition to formal employment. With a lack of options, it is necessary to ensure that the waste sector provides opportunities for decent work to enable workers to lift themselves out of poverty.