The Austrian network of isotopes in rivers comprises about 15 sampling locations and has been operated since 1976. The Danube isotope time series goes back to 1963. The isotopic composition of river water in Central Europe is mainly governed by the isotopic composition of precipitation in the catchment area; evaporation effects play only a minor role. Short-term and long-term isotope signals in precipitation are thus transmitted through the whole catchment. The influence of climatic changes has become observable in the long-term stable isotope time series of precipitation and surface waters. Environmental H values were around 8 TU in 2015, short-termH pulses up to about 80 TU in the rivers Danube and March were a consequence of releases from nuclear power plants. The complete isotope data series of this network will be included in the Global Network of Isotopes in Rivers database of the International Atomic Energy Agency (IAEA) in 2017. This article comprises a review of 50 years isotope monitoring on rivers and is also intended to provide base information on the (isotope-)hydrological conditions in Central Europe specifically for the end-users of these data, e.g. for modelling hydrological processes. Furthermore, this paper includes the 2006-2015 supplement adding to the Danube isotope set published earlier.

The hydrological application of isotope-ratio measurements is based upon variations of 2 H, 3 H and 18 O in the natural water cycle. Main targets of isotope-hydrological projects are the investigation of hydrological relationships, the hydrogeological situation, the residence times of groundwater, and the possible transport of pollutants. Isotope-hydrological data also play an important role in climate research. A good knowledge of the "isotopic environment" is necessary for the interpretation of isotope data. For this purpose, basic isotope data of all parts of the hydrological cycle have been gathered at the Vienna Arsenal for nearly fifty years. The emphasis was on the isotope ratios in precipitation as they represent the input values for isotopic investigations in the terrestrial part of the water cycle. The influence of climatic changes has become observable in the long-term isotope time series of precipitation and surface waters. This influence must also be taken into account when using isotope data from a longer time period in hydrology. Environmental 3 H values were around 10 TU in 2010, while slightly higher values can be observed in hydrological systems which still contain significant water portions from the sixties and seventies. Aside from a survey of the development of the "isotopic environment" in the last decades and a few key results, which helped to understand (isotope)hydrological processes, this paper includes some data sets meant for application purposes.

Results of stable isotope measurements (δ(2)H, δ(18)O) of daily grab samples, taken from the Danube River at Tulln (river km 1963) during 2012, show seasonal and short-term variations depending on the climatic/hydrological conditions and changes in the catchment area (temperature changes, heavy rains and snow melt processes). Isotope ratios in river water clearly reflect the isotopic composition of precipitation water in the catchment area since evaporation influences play a minor role. Average δ(2)H and δ(18)O values in 2012 are-78‰ and-11.0‰, respectively, deuterium excess averages 10‰. The entire variation amounts to 1.8‰ in δ(18)O and 15‰ in δ(2)H. Quick changes of the isotopic composition within a few days emphasise the necessity of daily sampling for the investigation of hydrological events, while monthly grab sampling seems sufficient for the investigation of long-term hydro-climatic trends. (3)H results show peaks (half-width 1-2 days, up to about 150 TU) exceeding the regional environmental level of about 9 TU, probably due to releases from nuclear power plants.

Rationale A silver phosphate reference material (Ag3PO4) for the measurement of stable oxygen isotope compositions is much needed; however, it is not available from the authorities distributing reference materials. This study aims to fill this gap by calibrating a new Ag3PO4 stable isotope comparison material produced by the University of Natural Resources and Life Sciences (BOKU). Methods Aliquots of Ag3PO4 were distributed to four laboratories who frequently measure the δ18O value in Ag3PO4; the University of Natural Resources and Life Sciences (BOKU), the University of Western Australia (UWA), the University of Helsinki (UH), and the Helmholtz Centre for Environmental Research (UFZ). The instruments used to perform the measurements were high‐temperature conversion elemental analysers coupled with continuous flow isotope ratio mass spectrometers. The working gas δ18O value was set to 0‰ and the normalization was done by a three‐point linear regression using the reference materials IAEA‐601, IAEA‐602, and NBS127. Results The mean δ18O value of the new BOKU Ag3PO4 comparison material on the VSMOW‐SLAP scale is 13.71‰ and the combined uncertainty is estimated as ±0.34‰. This estimated uncertainty is within the range typical for comparison materials of phosphates and sulphates. Consistent results from the different laboratories probably derived from similar instrumentation, and use of the same reference materials and normalization procedure. The matrix effect of the different reference materials used in this study was deemed negligible. Conclusions The BOKU Ag3PO4 can be used as an alternative comparison material for stable oxygen isotope analysis and is available for stable isotope research laboratories to facilitate calibration.

<p>Use of cover crops, mulches and other soil management practices have been widely promoted within the EU, as measures to draw down carbon dioxide and increase soil organic carbon in the fight against climate change. These approaches are being investigated in a number of programs and projects EU-wide, for example the EJP-SOIL program.</p><p>We used a long-term fully replicated maize based field trial with different crop and soil management practices, namely residue incorporation and/or inclusion of a cover crop, to explore carbon sequestration potential. We used natural abundance stable isotope approaches to follow the fate and residence time of mulched residues and to determine the most stable organic matter pools in these systems. We measured isotope signatures in particulate organic matter (POM), mineral associated organic matter (MOAM), stable aggregate bound organic matter (IA-POM) and soil microbial biomass (Mi-BIO) to characterize the impact of the soil fraction, on the fate and stability of the carbon pool and to test a number of emerging paradigms in soil science. We hypothesized that the residence time of the POM fraction and MOAM fractions would be lower under higher nitrogen inputs and that it would be possibly to detect these shifts using a stable isotope approach. Moreover, we used handheld and unmanned aerial vehicle UAV-captured multispectral data to investigate impacts of management on a plot scale.</p><p>Preliminary results show that even at low mulch rates (<3 t plant material ha<sup>-1</sup>) mulching significantly increased soil carbon storage in the long term, albeit at lower rates than predicted. This increase was due to increases in SOM in the top-soil. As hypothesized mulch with lower C:N ratios contributed less to overall soil carbon storage, but whether this was due to differences in carbon accumulation in the short-term POM pool or long-term MOAM pool remains to be revealed by on-going isotope analysis. The high replication and detailed investigation of this long term field trial should allow us to tease out a number of processes in the carbon and nitrogen cycle and allow us recommend suitable management practices for increasing soil organic carbon stocks. </p>