Pinholin
S2168 is an essential part of the phage Φ21
lytic protein system to release the virus progeny at the end of the
infection cycle. It is known as the simplest natural timing system
for its precise control of hole formation in the inner cytoplasmic
membrane. Pinholin S2168 is a 68 amino acid integral membrane
protein consisting of two transmembrane domains (TMDs) called TMD1
and TMD2. Despite its biological importance, structural and dynamic
information of the S2168 protein in a membrane environment
is not well understood. Systematic site-directed spin labeling and
continuous wave electron paramagnetic resonance (CW-EPR) spectroscopic
studies of pinholin S2168 in 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) proteoliposomes are used to reveal
the structural topology and dynamic properties in a native-like environment.
CW-EPR spectral line-shape analysis of the R1 side chain for 39 residue
positions of S2168 indicates that the TMDs have more restricted
mobility when compared to the N- and C-termini. CW-EPR power saturation
data indicate that TMD1 partially externalizes from the lipid bilayer
and interacts with the membrane surface, whereas TMD2 remains buried
in the lipid bilayer in the active conformation of pinholin S2168. A tentative structural topology model of pinholin S2168 is also suggested based on EPR spectroscopic data reported
in this study.
The mechanism for the lysis pathway of double-stranded DNA bacteriophages involves a small hole-forming class of membrane proteins, the holins. This study focuses on a poorly characterized class of holins, the pinholin, of which the S 21 protein of phage φ21 is the prototype. Here we report the first in vitro synthesis of the wildtype form of the S21 pinholin, S 21 68, and negativedominant mutant form, S 21 IRS, both prepared using solid phase peptide synthesis and studied using biophysical techniques. Both forms of the pinholin were labeled with a nitroxide spin label and successfully incorporated into both bicelles and multilamellar vesicles which are membrane mimetic systems. Circular dichroism revealed the two forms were both >80% alpha helical, in agreement with the predictions based on the literature. The molar ellipticity ratio [θ] 222 / [θ] 208 for both forms of the pinholin was 1.4, suggesting a coiled-coil tertiary structure in the bilayer consistent with the proposed oligomerization step in models for the mechanism of hole formation. 31 P solid-state NMR spectroscopic data on pinholin indicate a strong interaction of both forms of the pinholin with the membrane headgroups. The 31 P NMR data has an axially symmetric line shape which is consistent with lamellar phase proteoliposomes lipid mimetics.
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Volatile anesthetics are compounds which are commonly used to induce a reversable loss of consciousness (LOC) in animals. The molecular mechanism of how anesthetics induce LOC is largely unknown. However, observations have been made which show that there are genetically-encoded traits which influence the effective concentration of anesthetics in the inducement of LOC. Despite this longterm observation, little progress has been made in identifying genes involved in anesthetic sensitivity. One reason for this is that many techniques to test anesthetic sensitivity are technically challenging and are inhibitory for high-throughput studies. Here we introduce a technique for testing volatiles and aerosols with positional recording (VAAPR), a method which allows for high-throughput testing of the effect of anesthetics and other aerosolized drugs using Drosophila. Using VAAPR we show that the enzyme phospholipase D (PLD) significantly shifts the concentration of diethyl ether, chloroform, and isoflurane needed to induce LOC in Drosophila. We also show that PLD is required for a paradoxical hyperactivity phenotype. We expect that this technique will allow for additional genes to be found which control anesthetic sensitivity as well as other behavioral phenotypes.
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