This manuscript provides a concise review of the combined imaging and chemical sensing (CICS) technique performed with imaging fiber chemical sensors (IFCSs) and represents the first demonstration of CICS using a disposable microscope objective chemical sensor (dMOCS). The dMOCS assembly comprises two pieces that clamp around an imaging system's objective and a third adjoining piece that positions a sensing layer onto a sample's surface and within the objective's field of view. The prototype layer was oxygen-sensitive and was fabricated using a luminescent ruthenium metal complex and a gas permeable polymeric membrane. O 2 -sensitive dMOCSs were characterized with respect to their imaging capabilities, sensitivity, and temporal response, and the feasibility of performing a high-content screening assay was demonstrated with preliminary measurements of oxygen dynamics from living cells. While IFCSs are a requisite for analyzing remote samples that cannot be brought to a microscope stage, for those samples that can, CICS with a dMOCS possesses advantages with respect to spatial resolution, ease of fabrication, cost, and viewing area.