This study was aimed at determining the antioxidant activity of
commercial grape juices in inhibiting
the copper-catalyzed oxidation of human low-density lipoproteins (LDL)
in vitro and at relating
this activity to the phenolic composition of the juices. This work
also evaluated the effect of vitamin
C on this antioxidant activity. When standardized to a total
phenolic concentration of 10 μM gallic
acid equivalents (GAE), samples of grape juices inhibited LDL oxidation
from 62 to 75%. White
grape juices inhibited LDL oxidation on the average by 72%,
Concord purple grape juice by 67%,
and grape juice blends (mixture of white and Concord grape juice) by
63%. Vitamin C had no
significant effect on the antioxidant activity of the grape juices
tested. The antioxidant activity of
Concord juice samples was related to their anthocyanin levels, while
that of the white grape juices
was related to their levels of flavan-3-ols and hydroxycinnamates, as
determined by HPLC. On the
basis of the same total phenolic concentration, the antioxidant
activity of grape juices toward LDL
oxidation was comparable to that of several California red wine.
However, based on their undiluted
total phenolic concentration, the Concord and blends of grape juices
had comparable activity to
that of the red wines, while the white grape juices were less
active.
Keywords: LDL oxidation; phenolic compounds; antioxidants; grape juice;
flavonoids; hexanal;
HPLC
The authors assessed the diet and exercise habits and perceived barriers to following a healthy lifestyle of 471 college students. Sixty percent of the participants were female and 31% had BMIs > 25. Breakfast was the most commonly missed meal and 63% of students snacked one to two times per day. Fifty-eight percent of participants ate vegetables and 64% ate whole or canned fruit less than once per day. Men consumed more soda and alcohol and used higher fat dairy, ate more meat, and ate fewer vegetables and fruits than women. Over half of the subjects rated their diet as poor or fair with “lack of time” listed as the number one barrier to eating well. Men exercised more frequently and at greater intensity than women and were more confident with their body image. The most common barrier to exercise was “lack of time.” The results of this study have implications for the design of general and specific diet and physical activity interventions among college students.
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