A monolayer of 2-amino-4,6-di(dodecylamino)-1,3,5triazine 1 transferred from barbituric acid solution onto a mica plate is observed by atomic force microscopy (AFM) to show regularly arrayed terminal methyl groups of 1 as a result of hydrogen-bond networking.We have recently reported multisite molecular recognition between multicomponent monolayers on water and flavin adenine dinucleotide (FAD) in water. 1 This result opened the possibility to control the alignment of component amphiphiles within a monolayer.2 We extended this concept one step further in the present study and examined extended hydrogen-bond network formation between a monolayer of 2-amino-4,6-di(dodecylamino)-l,3,5-triazine 17 and aqueous barbituric acid.