The wound healing process was histopathologically investigated in a rabbit femoral artery (n = 48) after transection using a laser bipolar dissector (LBD, 1064nm the Nd:YAG bipolar contact laser) with laser power of 13W in 0.5-sec pulses. The cutting edges were harvested immediately (n = 6) and at 1 (n = 6), 4 (n = 6), and 7 (n = 6) days, 2 (n = 6), 3 (n = 6), and 4 (n = 6) weeks, and 6 months (n = 6), and evaluated by light microscopy. During the postoperative period, no perforation was seen in the 48 transected sites with the LBD. The healing process after LBD transection was accomplished within 2 weeks by organization of the intraluminal thrombus and by the formation of granulation tissue outside the adventitia of vessels. Characteristic morphological changes after LBD transection were recognized in the tapering area, i.e., cartilage and bone formation. Cartilaginous foci (n = 6) were observed in the media at 4 weeks after transection, and osseous foci (n = 3) in subendothelial space at 6 months. These observations may suggest that the heterotopic cartilaginous and osseous metaplasia of a vessel wall may be a result of the biostimulative effects of the Nd:YAG laser.
The optical laser setting for successful hemostatic transection of vessels using Laser Bipolar Dissector (LBD) and the important factor in closure and the healing process of the vessel stump were examined in abdominal aortas, mesenteric arteries and femoral arteries of rabbits (n=40).Interluminal bursting pressures (BP) were measured. The cutting edges were harvested at one week (n=4), at 2, 3 and 4 weeks (n=4) and 6 months (n=4), and evaluated by light microscopy.Histopathological examinations were carried out by HEand EVG stainings.The BP immediately after transection at laser power of 10 (n,=5) and 13W (n=5) was above 346 mmHg, and that of 15W (n=5) and 20W (n=5) was 280mmHg, 208 mmHg respectively. The transected sites of vessels revealed well-apposed tissue welding of vascular wall. The welding of internal elastic lamina without fragmentation is histologically most important for closure of the vessel stump. An increase in the laser power from 10 to 20W resulted in increased severity of histopathological deterioration of tissue connectivity (n=10, p<0.01) and in decreased bursting strength (n=5, p<0.01). Laser power of 10 to 13W was the most suitable for hemostatic dissection of large vessels. In the healing process, cartilaginous foci (n=4) were observed in the vascular cutting edges at 4 weeks after LBD dissection, and osseous foci (n=---3) were observed at 6 months.These observations may suggest that use of the LBD for vascular transection results in excellent tissue welding of vascular stumps in 2 to 3mm diameter arteries and that the the vascular stump is repaired with the strengest tissue material.
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