Avian pathogenic Escherichia coli (APEC) is ac ommon bacterial pathogen infecting chickens,r esulting in economic losses to the poultry industry worldwide.I np articular,APEC O1, one of the most common serotypes of APEC, is considered problematic due to its zoonotic potential. Therefore,m any attempts have been made to develop an effective vaccine against APEC O1. In fact, the lipopolysaccharide (LPS) O-antigen of APEC O1 has been shown to be apotent antigen for inducing specific protective immune responses. However,the detailed structure of the O-antigen of APEC O1 is not clear.T he present study demonstrates the first synthesis of ap entasaccharide repeating unit of LPS derived from virulent E. coli O1 and its conjugate with BSA. ELISA tests using the semi-synthetic glycoconjugate and the APEC O1 immune chicken serum revealed that the pentasaccharide is aglycotope candidate of APEC O1, with great potential as an antigen for vaccine development.

Avian pathogenic Escherichia coli (APEC) is ac ommon bacterial pathogen infecting chickens,r esulting in economic losses to the poultry industry worldwide.I np articular,APEC O1, one of the most common serotypes of APEC, is considered problematic due to its zoonotic potential. Therefore,m any attempts have been made to develop an effective vaccine against APEC O1. In fact, the lipopolysaccharide (LPS) O-antigen of APEC O1 has been shown to be apotent antigen for inducing specific protective immune responses. However,the detailed structure of the O-antigen of APEC O1 is not clear.T he present study demonstrates the first synthesis of ap entasaccharide repeating unit of LPS derived from virulent E. coli O1 and its conjugate with BSA. ELISA tests using the semi-synthetic glycoconjugate and the APEC O1 immune chicken serum revealed that the pentasaccharide is aglycotope candidate of APEC O1, with great potential as an antigen for vaccine development.

Identification of the glycotope … …o fa vian pathogenic E. coli (APEC) O1 is important for the design of an ew generation of improved and safer vaccines.I nt heir Research Article on page 1789, Daisuke Takahashi, Kazunobu Toshima, and co-workers synthesize the unique pentasaccharide derived from virulent E. coli O1 and reveal that the pentasaccharide is ag lycotope candidate of APEC O1, with great potential as an antigen for vaccine development.

Die Identifizierung des Glykotops … …d es aviären pathogenen E. coli (APEC) O1 ist wichtig fürd ie Entwicklung einer neuen Generation von verbesserten und sicheren Impfstoffen. In ihrem Forschungsartikel auf S. 1817 synthetisieren Daisuke Takahashi, Kazunobu Toshima et al. das vom virulenten E. coli O1 abgeleitete Pentasaccharid und zeigen, dass das Pentasaccharid ein Glykotop-Kandidat von APEC O1 ist, mit großem Potenzial als Antigen fürd ie Impfstoffentwicklung.