The existence and distribution patterns of microsatellites in the tiger puffer genome were evaluated utilizing DNA databank entries. Microsatellite regions amounted to only 0.96% of the 22.5 million bp included in the tiger puffer database. Di-nucleotide repeats were most common (90.1% of all microsatellites). Twelve microsatellite loci were isolated from the entries. Analysis of genetic polymorphism at these loci in the wild tiger puffer (n = 50) revealed a high degree of length polymorphisms, in which the number of alleles per locus ranged from 11 to 34. The expected heterozygosity was between 0.869 and 0.953. Mendelian inheritance was demonstrated in all but two loci (which seemed to include null alleles) by analysis of genotype ratios in the offspring of a single captive. Probability of identity of all loci ranged from 0.007 to 0.028. Band sharing index was low (0.119) in wild tiger puffer and high in the offspring of the captive (0.525). These results indicate the usefulness of the microsatellite markers identified for future population analyzes in tiger puffer.KEY WORDS: band sharing index, DNA database, fugu, microsatellite, null allele, Takifugu rubripes, tiger puffer, sample size.

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Thanks to the open technologies around the world wide web, the authors as just college teachers have been able to develop a compact and practical online quiz system we call QDB to satisfy needs in their classes. In this paper, first we introduce our system to give ideas what the system can do. Then we show the effectiveness of the system by describing the effects of practical uses of the system in our classes for years including the effect of the recently added retry-functionality. Then as an example of the feasibility of our development we explain how simple to implement the retry-functionality because of the compactness of the system and again the great power the open technologies give us and show the speed of the system. As conclusion we suggest possibilities of a compact and flexible system as a form (or a part) of elearning systems made to your order.

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