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Biosensing has capitalized on the excellent characteristics and properties of nanostructures for detecting glucose levels in diabetic patients. In glucose sensing systems, the fabrication of a suitable matrix for immobilizing glucose oxidase (GOx) has become more interesting for the application of nanofibers in enzymatic electrochemical biosensors. These nanofiber based electrochemical biosensors are superior in manufacturability and performance due to low cost, diversity of materials, ease of miniaturization, response time, durability, and structure versatility. This perspective highlights the latest material integration of various nanofibrous composite membranes of carbon nanotubes, carbon nanofibers, conductive nanoparticles and conductive polymers, that provide large matrix-like, porous surfaces to enhance the immobilization of enzymes, for the fabrication of glucose biosensors.

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