No abstract

Potassium is an extracellular ion that plays an important role in the electrophysiological function of the heart. Any change in the extracellular concentration of potassium can have a marked impression upon cardiac electrophysiology. Underlying kidney disease, certain medical conditions, dietary indiscretions, and medications can precipitate hyperkalemia. Drug-induced hyperkalemia is one of the most important causes of increased serum potassium in everyday clinical practice. Hyperkalemia can lead to various lifethreatening dysrhythmias and if left untreated, it will ultimately cause ventricular arrhythmias and asystole. This case report describes an end-stage renal disease (ESRD) patient taking atenolol who presented with hyperkalemia and type II second degree atrioventricular (AV) block. He presented with hyperkalemia when atenolol was introduced and normalized when atenolol was discontinued. The heart block completely resolved after treatment of hyperkalemia.

This study aimed to isolate indigenous Antagonistic Bacteria (AB) against common soil-borne phytopathogens, including Rhizoctonia solani, Phythium sp., Fusarium oxysporum. Biosynthesis of Indole-3-Acetic Acid (IAA), generation of Hydrogen Cyanide (HCN), and siderophores production were assessed for their involvement in the antagonistic activities. Rhizospheric soil of bean roots, sunflowers, wheat, rice, and humic and semi flooded soils were used to isolate twenty-one bacterial strains for phytopathogenic antagonism. It was found that nine isolates have potential antagonistic activity against three common soilborne pathogens. Antifungal productivity for IAA, HCN, and siderophores was screened on the isolates while nine ABs were identified. To choose statistically significant isolate for the formulation, the principal component analysis was performed with five variables (IAA production, siderophores production index, antagonistic activities against three phytopathogens). Among the nine isolates, the isolate Pseudomonas alicaligenes shew a positive correlation with all variables. In particular, the strain demonstrated to be an antagonistic strain against the fungal pathogens and a strong producer of siderophores, HCN and IAA, We prepared the biocontrol agents with rice flour, glutinous rice flour, Monosodium Glutamate and Chitosan that were found to maintain the up to three months for convenience of field applications.

Rhizospheric bacteria are naturally occurring soil microbes that are aggressively found in the plant rhizosphere, at root surface and in association with roots. They give satisfactory benefit plants by several mechanisms such as nitrogen fixation, phosphate solubilization, potassium decomposition, IAA production, antagonism against phytopathogenic microorganisms by production of siderophore, antibiotics and cell wall degrading enzymes. The total number of beneficial bacteria were isolated from different rhizospheric soil in agricultural lands. The isolated bacterial strains were screened for their plant growth promoting factors such as production of ammonia, siderophore, cellulase, chitinase, and pectinase enzyme. All of the isolates produced ammonia and 79% of the isolates produced siderophore on chrome azurole S agar plates. Furthermore, the bacterial isolates produced cell wall degrading enzyme; pectinase (69%), cellulase (94%), chitinase (51%), amylase (61%) and glucanase enzyme (59%) on agar plate method. The isolates also produced auxin type plant hormone (IAA), all the isolates produced IAA and the highest IAA producing strain is W1 and the produce amount was 21.91mg/L. Among the isolated bacteria, only two strains could produce HCN with the use of Feigl-Anger paper method. The recent study suggests that the use of these PGPR isolates as inoculants might be a promising source for sustainable agricultural use.

Banana propagation is dependent on propagation by tissue culture for industrial purposes. Due to lack of native endophytes in tissue culture plantlets, reintroduction of beneficial microorganisms to tissue culture plantlets become popular as a useful foundation for improving the level of establishment, increasing plant growth parameters, protecting the plantlets against pest and diseases and overall performance for field plantation. In the present study, rhizospheric and endophytic four bacterial species (Pseudomonas fluorescens, Bacillus putida, Bacillus pumilus, Bacillus amyloliquefaciens were isolated from different parts of healthy banana plants such as root, leaf and pseudostem. Banana tissue culture plantlets were cultured for root formation stage on MS medium and used as host for artificial inoculation. Then, bacterial inoculation was carried out with tissue culture plantlets both in vitro and ex vitro conditions. All banana plantlets used for this experiment were successfully acclimatized and survived in the greenhouse. The experiment consisted of three treatments: (1) bacterial inoculum with in vitro rooted plantlets for 2 weeks (2) bacterial inoculum with ex vitro (hardening) plantlets (3) control with sterilized distilled water. Among these treatments, in vitro inoculation affected significantly increased plant height, number of leaves and plant girth for all data collection times with the highest increment indices: (24.0, 7.0, and 7.3). Ex vitro inoculation showed the second highest increment indices: (22.0, 6.9 and 6.3) for plant height, number of leaves and plant girth, respectively. The lowest number of plant growth parameters were observed in control treatment for all four data collection time (19.0, 6.2, and 6.0). According to the results, application of mixtures of bacterial inoculum was effective for enhanced plant growth parameters of tissue culture banana plantlets under greenhouse condition. In addition, two weeks artificial inoculation during in vitro plantlets stage was found the most suitable method for application of microbes with tissue culture banana plantlets which can promote the microbial rhizosphere of banana prior to field plantation.