A novel countercurrent chromatographic technique is described for the preparative separation-with excellent resolution-of up to multigram quantities of organic acids. This separation is accomplished by addition of a simple acid (e.g., trifluoroacetic acid) to the sample solution or stationary phase, followed by isocratic elution with a basic mobile phase. The acids elute as well-resolved rectangularly shaped peaks. This technique should find widespread application in any organic chemistry laboratory for the separation of crude mixtures and for the purification of isolated acids.

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A highly sensitive high performance liquid chromatographic method with fluorometric postcolumn labeling using 2-cyanoacetamide was developed for determination of unsaturated disaccharides produced enzymatically from chondroitin sulfates (ChS). The calibration curves of the unsaturated disaccharides were linear over the range from 2 pmol to 5 nmol. This method was applied to the analysis of normal rabbit plasma for ChS to verify the sensitivity and practicality.

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