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Abstract1‐Carboxy‐N,N‐dimethyl‐N‐(2′‐methacryloyloxyethyl)methanaminium inner salt (CMB) was polymerized by ATRP initiated with a disulfide difunctionalized by 2‐bromoisobutyryl groups. The disulfide‐carrying carboxybetaine polymer (DT‐PCMB) was used for the preparation of PCMB‐protected gold nanoparticles (PCMB‐AuNPs) obtained by the reduction of hydrogen tetrachloroaurate (HAuCl4) in the presence of the DT‐PCMB of different molecular weights at different molar ratios of DT‐PCMB and HAuCl4. The sizes of gold cores in the PCMB‐AuNPs tended to increase upon decreasing concentration and molecular weight of the DT‐PCMB. The PCMB‐AuNPs possessed a high dispersion stability, and showed a resistance against non‐specific adsorption of proteins (bovine serum albumin, human serum albumin, lysozyme, and cytochrome c). Therefore, DT‐PCMB is a quite suitable stabilizing ligand to prepare inert AuNPs and the PCMB‐AuNPs will be useful in biomedical applications.magnified image

3-Sulfo-N,N-dimethyl-N-(2'-methacryloyloxyethyl)propanaminium inner salt (SPB) was polymerized on a glass plate with a surface-confined initiator of atom transfer radical polymerization (ATRP) having a 2-bromoisobutyryl group. The glass plate modified with a brush of sulfobetaine telomer (PSPB) was highly hydrophilic and showed a strong resistance against nonspecific adsorption of proteins such as lysozyme and albumin. Through the polymerization from the free surface of PSPB chain by ATRP, furthermore, N-methacryloyloxysuccinimide (MAOSu) residues were introduced, and the incubation of the telomer (PSPB-b-PMAOSu)-modified glass chip with a lectin (concanavalin A, Con A) gave a glass chip covered with the Con-A-modified PSPB brush. The Con A fixed to the zwitterionic telomer brush pursued specific binding of mannose residues accumulated on the surface of Au colloidal particles, resulting in the increase in absorbance at 550 nm ascribable to localized surface plasmon resonance, while the nonspecific adsorption of proteins to the surface of the glass chip was still largely suppressed. The present results indicate usefulness of the zwitterionic telomer surface with antibiofouling properties as a scaffold for specific sensing devices.

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