wi 0 phase ratio respectively. volume fraction of the binary solvent containing Subscripts m and s represent mobile phase and solute, the organic Si in the complex mixture APPENDIXThe discussion will be limited to ternary solvents. It is assumed that they are prepared by mixing the binary systems BS1 and BSz composed of water and the organic modifier SI and water and the organic modifier Sz, respectively. The volume fraction of Si in BSi is x i , the volume fraction of Si in the ternary is X i and the volume fraction of BSi in the ternary is win Taking into account the fact that w1 + wz = 1, it can be written that(1 -w1)xz = xz (18) If the composition of one of the binary systems is fixed (for instance xl), then the composition of the other binary mixture and the value of w1 can be calculated by using the following equations:x 1 > x1 (21) (22) Equation 20 precludes that and x1 > X d ( 1 -XZ) Registry No. MeOH, 67-66-1; MeCN, 75-05-8; THF, 109-99-9; dioxane, 123-91-1. LITERATURE CITED Jandera, P.; Colin, H.; Guiochon, G. Anal. Chem. lD82, 54, 435-441. Colln, H.; Krstulovic, A.; Yun, 2.; Gulochon, Q. J . Chromatogr., In press. Colin, H.; Guiochon, Q,; Jandera, P. Chromatographla, in press. Snyder, L. R.; Klrkland, J. J. I n Introduction to Modern Liquid Chromatography", 2nd ed.; Wiley-Interscience: New York, 1979. Glaich, J. L.; Kirkland, J. J.; Squire, K. M. J. Chromatogr. lD80, 199, "Handbook of Chemistry and Physics", 61st ed.; CRC Press: Boca 57-79. Raton, FL, 1980. McCormlck, R. M.; Karger, B. L. Anal. Chem. ID81, 52, 741-744. Colin, H.; Schmlner, J. M.; Guiochon, G. Anal. Chem. lD81, 53, 625-632.The retentlon behavior of some aromatic compounds on silica gels wlth chemically bonded cyclodextrin molecules has been studied. The capacity factor of the sample generally increased by virtue of the specific Interaction between cyclodextrin units and sample molecules. The effects of (a) the substituents and thelr relative positlon on the benzene or naphthalene ring, (b) the spacer length, (c) the mobile phase, and (d) the variety of cyclodextrin were examlned. The parameters of the mobile phase were the kind of organic solvent used and the water content. The effect of addltlon of cyclodextrin to the mobile phase was also Investigated under conventional reversed-phase hlgh-performance liquid chromatography conditions In order to confirm the existence of inclusion equilibrium between cyclodextrln and sample molecules.Cyclodextrins (CD's), which are known to be cyclic oligosaccharides consisting of six or more a-( 1,4)-linked D-glucopyranose units, form inclusion complexes with a variety of organic molecules both in the solid state and in an aqueous solution. The stability of such inclusion complexes is, in general, most closely related to the fitness of the size of guest molecules to that of the cavity of cyclodextrin units, although many other factors such as van der Waals forces, dipole-dipole interaction, hydrogen bonding, and hydrophobic interaction
