ABSTRACT:The indiscriminate discharge of Cassava mill effluent pose serious environmental and public health implications. This work was aimed at assessing the biodegradation potentials of indigenous microbial isolates from cassava mill effluent using screening and shake flask degradation tests. The mean heterotrophic bacterial and fungal counts were 3.4±0.26 x 10 6 cfu/ml and 1.1±0.20 x 10 6 cfu/ml respectively. The microorganisms isolated, characterized and identified include the genus Pseudomonas (15.48%), Bacillus (12.41%), Acetobacter and Rhizopus (10.88%), Corynebacterium (9.33%), Lactobacillus, Micrococcus and Aspergillus (7.79%), Staphylococcus and Penicillium (6.25%) and Saccharomyces species (4.62%). The pH (5.39 ± 0.14), electrical conductivity (2506.