The fluorochrome Uvitex 2B [4,4-BIS(2-di(2-hyroxyethyl)-amino-4-(3-sulfophenylamino)-1,3,5-triazine-6-ylamino )-stilbene-2,2-disulfonic acid, sodium salt] stain was used for detection of Microsporid ium seriolae (Protozoa: Microspora), the causative agent of beko disease of yellowtail Seriola quinquera diata and goldstriped amberjack S. lalandi juveniles. For detection of the parasite, microscopical fluorescence examination for spores in the Uvitex 2B-stained smears of trunk muscle homogenates was much more sensitive than the conventional visual inspection for "cysts" in the trunk muscle. The Uvitex 2B-H & E stain of deparaffinized sections was applicable to the examination for the sporulation sequence in "cysts" and spore dispersal into adjacent tissues or other organs. Thus, Uvitex 2B stain was found to be useful not only for a rapid and sensitive diagnosis of beko disease but also for histopathological studies of microsporidian infections.

No abstract

No abstract

Trichlorobenzenes can be easily decomposed by alternating current electrolysis in aqueous solution. The mechanism of the decomposition was found to be based on selective redox reactions with different radicals—hydrogen atoms and hydroxyl radicals—generated by water electrolysis.

No abstract