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The bonding behavior between hydrophobically modified alkaline-treated gelatin (hm-AlGltn) films and porcine blood vessels was evaluated under wet conditions. Hexanoyl (Hx: C6), decanoyl (Dec: C10), and stearyl (Ste: C18) chlorides were introduced into the amino groups of AlGltn to obtain HxAlGltn, DecAlGltn, and SteAlGltn, respectively, with various modification percentages. The hm-AlGltn was fabricated into films and thermally crosslinked to obtain water-insoluble films (t-hm-AlGltn). The 42% modified t-HxAlGltn (t-42HxAlGltn) possessed higher wettability than the 38% modified t-DecAlGltn (t-38DecAlGltn) and the 44% modified t-SteAlGltn (t-44SteAlGltn) films, and the t-42HxAlGltn film showed a high bonding strength with the blood vessel compared with all the hm-AlGltn films. Histological observations indicated that t-42HxAlGltn and t-38DecAlGltn remained on the blood vessel even after the bonding strength measurements. From cell culture experiments, the t-42HxAlGltn films showed significant cell adhesion compared to other films. These findings indicate that the Hx group easily interpenetrated the surface of blood vessels and effectively enhanced the bonding strength between the films and the tissue.

The bonding behavior was determined for hydrophobically modified alkaline-treated gelatin on wet porcine intestinal surfaces. The modified gelatin films were obtained by reacting the amino groups of alkaline-treated gelatin with fatty acid chlorides of different alkyl chain lengths, namely, hexanoyl (Hx: C6) chloride, decanoyl (Dec: C10) chloride, and stearoyl (Ste: C18) chloride. Three kinds of the films were prepared, 32HxAlGltn, 24DecAlGltn, and 26SteAlGltn that had substitution ratios of hydrophobic groups to the amino groups of 32HxAlGltn, 24DecAlGltn, and 26SteAlGltn of 32%, 24%, and 26%, respectively. The 32HxAlGltn film had the strongest bonding to porcine intestinal surfaces. A thick 32HxAlGltn film remained on the intestinal surface even after the bonded film was scraped off for the measurement of bonding strength. In addition, the burst strength increased with an increase in the substitution ratio of the Hx group. Thus, the HxAlGltn film with the higher Hx modification ratio has a potential as a sealant material to prevent agglutination of intestinal surfaces.

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