The substrate specificities of two pentose isomerases have been determined for the aldose substrates. r>-Xylose isomerase is shown to be specific for a-~-xylose or a-D-glucose. arabinose isomerase uses /3-L-arabinose only. Inversion of the anomeric center of the aldopyranose substrates does not seem to be catalyzed by these enzymes. The anomeric specificities of these enzymes and of phosphoglucose
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