No abstract

Nine different arboviruses are known to be transmitted by, or associated with, mosquitoes in Europe, and several (West Nile, Sindbis and Tahyna viruses) are reported to cause outbreaks of human disease. Although there have been no reported human cases in Great Britain (GB), there have been no published in-depth serological surveys for evidence of human infection. This paper investigates the ecological and entomological factors that could influence or restrict transmission of these viruses in GB, suggesting that in addition to West Nile virus, Sindbis and Tahyna viruses could exist in enzootic cycles, and that certain ecological factors could facilitate transmission to humans. However, the level of transmission is likely to be lower than in endemic foci elsewhere in Europe due to key ecological differences related to spatial and temporal dynamics of putative mosquito vectors and presence of key reservoir hosts. Knowledge of the potential GB-specific disease ecology can aid assessments of risk from mosquito-borne arboviruses.

Outbreaks of West Nile virus (WNV) infection have occurred sporadically in Europe, apparently due to the migration of infected birds and the subsequent establishment of a transmission cycle involving culicine and anopheline mosquitoes. Both human and equine species become infected, but are considered as dead end hosts since they play an insignificant role in the maintenance of the cycle. Following the introduction of WNV into the United States in 1999 it is increasingly apparent that the virus has an extraordinary ability to infect a very broad range of arthropod species. Here we consider the potential for British mosquitoes to transmit WNV in the event that it is introduced into the UK.

The life-history of I. pacificus when fed on guinea-pigs under laboratory conditions is described. Feeding of the female proceeds for 10–11 days. Between 120 and 191 mg of concentrated food is found in the female's gut on detaching from this host, and this is directly related to the unfed body weight of the tick.The egg-laying pattern, in general, shows an initial peak, after which the number of eggs tails off, the whole process lasting approximately 33–40 days. The number of eggs laid by females varies between 790 and 1300. The preoviposition period is about 11–16 days.Larvae hatch in about 53–55 days, and their feeding time ranges from 4 to 9 days, although in the present experiments larvae emerging from early laid eggs took about 10 days to complete engorgements.Moulting of larvae to nymphs at 90% R.H. took 37–38 days, and nymphs fed for 7–11 days, with most becoming replete on the ninth.Very few females emerged from these nymphs, and those that did emerged in just over 30 days. The reasons for this are not clear. No diapause appeared to be necessary to complete the life-cycle, and with due allowance for hardening of the mouthparts the life-history of one generation was about 7 months. Females died within 3 or 4 months if hosts were not available.From the literature and from hitherto unpublished records some 55 vertebrates have been implicated as hosts to I. pacificus; these hosts include a wide range of mammals, six species of birds and possibly nine species of lizards.The species has been listed from British Columbia and from five western states of U.S.A. The need for more intensive ecological work on this species is emphasized.We are indebted to Glen M. Kohls, J. D. Gregson and D. E. Johnstone for permission to use unpublished records from their respective institutions. One of us (D.R.A) is also indebted to the Wellcome Trustees for financial assistance to visit Canada during the preliminary stages of the work.

1. Eggs of the British woodland mosquito, Aedes puncror (Kirby), were subjected to a variety of media in an attempt to determine the hatching stimulus.2. It was found that continuous immersion in distilled water evoked almost no hatching (0.2%); intermittent removal from the water gave low hatching (8.9%).3 . Slow deoxygenation of the medium by either bacterial growth or the introduction of nitrogen produced the highest level of hatching (26.0-90.0%). Eggs of A.punctor were shown to hatch within a 4 h period of the oxygen concentration in the medium reaching zero.