Background: As opioid overdose deaths climb, legislation supporting pharmacists in developing their role to address the crisis has expanded. Although Minnesota pharmacists are encouraged to utilize opiate antagonist, syringe access and authorized collector legislation, the use patterns of these tools are unknown. Methods: A mixed-methods approach was used to survey 8405 Minnesota-licensed pharmacists on their practices related to the opioid crisis. An analysis of community pharmacist utilization of opioid-related legislation was conducted. Results: The majority (88.64%) of respondents indicated that they had not dispensed naloxone in the past month using a protocol; 59.69% reported that they had not dispensed naloxone by any method in the past month. Over sixty percent (60.61%) of respondents agreed they are comfortable with dispensing syringes and would dispense noninsulin syringes in their pharmacy under the statewide Syringe Access Initiative; 25.86% reported that they are not comfortable dispensing syringes. The majority (78.64%) of respondents reported that they do not participate in collecting unwanted pharmaceuticals. Conclusion: While pharmacists have the potential to play a key role in efforts focused on addressing the opioid crisis through harm reduction strategies, this role and the use of supporting legislation is currently underutilized in the state of Minnesota.

Introduction Although the opioid crisis has been acknowledged as a public health emergency that requires collaboration and engagement from pharmacists, the perceptions of pharmacists on their role in harm reduction strategies and their views on opioid use have not been well established. Objectives The primary objective of this study was to understand Minnesota pharmacists' attitudes about the profession's role in the opioid crisis, including perceptions on the pharmacist's public health and clinical roles within the crisis, views on the dispensing of naloxone, and attitudes toward harm reduction strategies including syringe access. Methods This was a mixed‐method study that utilized an anonymous email survey containing Likert‐type responses which were quantitatively analyzed, as well as open comment boxes evaluated using Consensual Qualitative Research (CQR) methods. Pharmacists with a Minnesota license from all practice settings were invited to participate in the survey. Results While the majority of participating pharmacists acknowledged a need within their communities to address the opioid epidemic agreeing that the profession should have a role in the community response, including provision of patient education and naloxone distribution, a small number of pharmacists believed that either the epidemic was not pertinent to their practice or pharmacy as a profession should not be involved. Concerns regarding naloxone included lack of support from other health professionals, time constraints in practice, and safety and liability concerns with naloxone. Conclusion Considerable opportunity for increased pharmacist education on both opioid use disorder and the role of the pharmacist in harm reduction initiatives exists. Pharmacists from a wide variety of clinical practice settings have appreciable potential to influence the opioid crisis by embracing their clinical and public health roles.

Tissue regeneration requires not only the replacement of lost cells and tissues, but also the recreation of morphologies and patterns. Skin pigment pattern is a relatively simple system that can allow researchers to uncover the underlying mechanisms of pattern formation. To gain insight into how pigment patterns form, undergraduate students in the senior level course Developmental Biology designed an experiment that assayed pigment patterns in original and regenerated caudal fins of wild-type, striped, and mutant, spotted zebrafish. A majority of the WT fins regenerated with a similar striped pattern. In contrast, the pattern of spots even in the original fins of the mutants varied among individual fish. Similarly, the majority of the spots in the mutants did not regenerate with the same morphology, size, or spacing as the original fins. This was true even when only a small amount of fin was removed, leaving most of the fin to potentially reseed the pattern in the regenerating tissue. This suggests that the mechanism that creates the wild-type, striped pattern persists to recreate the pattern during regeneration. The mechanism that creates the spots in the mutants, however, must include an unknown element that introduces variability.