BackgroundSerial block face scanning electron microscopy (SBFEM) is becoming a popular technology in neuroscience. We have seen in the last years an increasing number of works addressing the problem of segmenting cellular structures in SBFEM images of brain tissue. The vast majority of them is designed to segment one specific structure, typically membranes, synapses and mitochondria. Our hypothesis is that the performance of these algorithms can be improved by concurrently segmenting more than one structure using image descriptions obtained at different scales.ResultsWe consider the simultaneous segmentation of two structures, namely, synapses with mitochondria, and mitochondra with membranes. To this end we select three image stacks encompassing different SBFEM acquisition technologies and image resolutions. We introduce both a new Boosting algorithm to perform feature scale selection and the Jaccard Curve as a tool compare several segmentation results. We then experimentally study the gains in performance obtained when simultaneously segmenting two structures with properly selected image descriptor scales. The results show that by doing so we achieve significant gains in segmentation accuracy when compared to the best results in the literature.ConclusionsSimultaneously segmenting several neuronal structures described at different scales provides voxel classification algorithms with highly discriminating features that significantly improve segmentation accuracy.

Abstract-Full understanding of the architecture of the brain is a long term goal of neuroscience. To achieve it, advanced image processing tools are required, that automate the the analysis and reconstruction of brain structures. Synapses and mitochondria are two prominent structures with neurological interest for which various automated image segmentation approaches have been recently proposed. In this work we present a comparative study of several image feature descriptors used for the segmentation of synapses and mitochondria in stacks of electron microscopy images.

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