[1] Two large-scale cross-hole pumping tests were conducted at depths of 191-226 m and 662-706 m in deep boreholes at the Mizunami Underground Research Laboratory (MIU) construction site in central Japan. During these two tests, induced groundwater responses were monitored at many observation intervals at various depths in different boreholes at the site. We analyze the two cross-hole pumping tests using transient hydraulic tomography (THT) based on an efficient sequential successive linear estimator to compute the hydraulic conductivity (K) and specific storage (S s ) tomograms, as well as their uncertainties in three dimensions. The equivalent K and S s estimates obtained using asymptotic analysis treating the medium to be homogeneous served as the mean parameter estimates for the 3-D stochastic inverse modeling effort. Results show several, distinct, high K and low S s zones that are continuous over hundreds of meters, which appear to delineate fault zones and their connectivity. The THT analysis of the tests also identified a low K zone which corresponds with a known fault zone trending NNW and has been found to compartmentalize groundwater flow at the site. These results corroborate well with observed water level records, available fault information, and coseismic groundwater level responses during several large earthquakes. The successful application of THT to cross-hole pumping tests conducted in fractured granite at this site suggests that THT is a promising approach to delineate large-scale K and S s heterogeneities, fracture connectivity, and to quantify uncertainty of the estimated fields.

[1] The accurate characterization of fractured geologic medium, imaging of fracture patterns and their connectivity have been a challenge for decades. Recently, hydraulic tomography has been proposed as a new method for imaging the hydraulic conductivity (K) and specific storage (S s ) distributions of fractured geologic media. While encouraging results have been obtained in the field, the method has not been rigorously assessed in a controlled laboratory setting. In this study, we assess the performance of transient hydraulic tomography (THT) in a fractured dolomitic rock block. The block is characterized through flow-through tests and multiple pumping tests. The pumping test data were then analyzed with the THT code of Zhu and Yeh (2005) to image the fracture patterns and their connectivity through the delineation of K and S s distributions (or tomograms). Results show that the THT analysis of pumping tests yields high-K and low-S s zones that capture the fracture pattern and their connectivity quite well and those patterns become more vivid as additional pumping test data are added to the inverse model. The performance of the estimated K and S s tomograms are then assessed by: (1) comparing the tomograms obtained from synthetic to real data; (2) comparing the tomograms from two different pumping configurations; (3) comparing the estimated geometric mean of the hydraulic conductivity (K G ) from the K tomogram to the effective hydraulic conductivity (K eff ) estimated from the flow-through tests; and (4) predicting five independent pumping tests not used in the construction of the K and S s tomograms. The performance assessment of the K and S s tomograms reveals that THT is able to image high-K and low-S s zones that correspond to fracture locations in the fractured rock block and that the tomograms can be used to predict drawdowns from pumping tests not used in the construction of the tomograms with reasonable fidelity.

Fracture zones and their connectivity in geologic media are of great importance to ground water resources management as well as ground water contamination prevention and remediation. In this paper, we applied a recently developed hydraulic tomography (HT) technique and an analysis algorithm (sequential successive linear estimator) to synthetic fractured media. The application aims to explore the potential utility of the technique and the algorithm for characterizing fracture zone distribution and their connectivity. Results of this investigation showed that using HT with a limited number of wells, the fracture zone distribution and its connectivity (general pattern) can be mapped satisfactorily although estimated hydraulic property fields are smooth. As the number of wells and monitoring ports increases, the fracture zone distribution and connectivity become vivid and the estimated hydraulic properties approach true values. We hope that the success of this application may promote the development and application of the new generations of technology (i.e., hydraulic, tracer, pneumatic tomographic surveys) for mapping fractures and other features in geologic media.

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Aim: Advanced glycation end products (AGE) and a receptor for AGE (RAGE) play a key role in diabetic vascular complications. Matrix metalloproteinases (MMPs) and apoptosis contribute to plaque instability. The renin-angiotensin system (RAS) is crucial for NADPH oxidase-dependent redox signaling pathways in the vascular wall. We investigated the effects of RAS blockade on AGE-triggered signaling pathways and its downstream events, including MMP-9 and apoptosis. Methods: We used cultured rabbit aortic smooth muscle cells (SMCs), which were stimulated with AGE in the presence or absence of temocaprilat or olmesartan. Results: Angiotensin converting enzyme (ACE) mRNA levels were increased 4 to 6 hours after adding AGE. AGE induced Rac1 and p47 phox membrane translocation, reactive oxygen species (ROS) generation and NF-B phosphorylation within 15 minutes, and various molecular expressions after 18 hours, which were attenuated by RAS blockade by temocaprilat or olmesartan. AGE-induced RAGE expression, as well as other molecules, including membrane type 1-MMP (MT1-MMP), monocyte chemoattractant protein-1 (MCP-1) and plasminogen activator inhibitor-1 (PAI-1), was NADPH oxidase signaling-dependent and blunted by temocaprilat and olmesartan. The parameters of plaque instability, including MMP-9 expression and activity, and apoptosis were up-regulated by AGE, which was markedly attenuated by temocaprilat or olmesartan. Using isolated human monocyte culture, AGE-induced ROS generation and molecular expression were also attenuated by RAS blockade.Conclusion: The present study shows that AGE-triggered NADPH oxidase signaling pathways, including MMP-9 and apoptosis, were attenuated by RAS blockade, which may be an attractive strategy for treating plaque instability in diabetic vascular complications. J Atheroscler Thromb, 2010; 17:590-600.