High-throughput techniques to evaluate the effect of ligand density for impurity separations with multimodal cation exchange resins Scale-down, high-throughput screening techniques are well on their way to becoming a commodity in downstream bioprocess development, especially for the rapid development of chromatography process steps. This work used both resin slurry plate and miniature column high-throughput screening methodologies to identify the best resin properties for mAb separations utilizing a multimodal chromatography ligand interaction. A ligand with both cation exchange and hydrophobic interaction properties was studied at several ligand densities and compared to a commercially available multimodal resin with a larger particle size at high ligand density. The resins were screened with mAbs containing distinct process impurities (aggregates and a hydrophobic variant), and optimized conditions provided more than a log of clearance of both types of impurities for the different resins screened. These studies reveal that while a smaller particle size is generally preferable, optimal ligand densities can be different depending on the properties of both the mAb and impurity studied.

Barlow, has long been associated with the Pioneer Health Centre Ltd, presently serving as its vice-chairman. He believes that the philosophy which underpinned the activities of George Scott Williamson, Innes Pearse, and their associates has been insufficiently appreciated by historians and health professionals alike. Accordingly, we have been pleased to allow him to expound that philosophy "from the inside", and hope that our readers will find it a convincing example of the kind of thinking that led to the Peckham experiment.] In October 1984, the Journal of the Royal Society of Medicine, commenting on a discussion paper by Dr Court,' reported that the Royal College of General Practitioners and the General Medical Services Committee of the British Medical Association are producing a record card for use in general practitioner and other clinics which undertake "developmental surveillance". The function of [the proposed] clinics is threefold. Firstly, there is the examination of all babies at appropriate intervals to see that they are growing normally ... and that such disabilities or potential disabilities as warrant intervention can be identified so that remedial action can be taken .... The most important tests are those directed at identifying congenital dislocation of the hips, squint, hearing loss and, in boys, maldescent or nondescent of the testes. Other tests are directed at identifying any defects in the four major functions; gross and fine motor, coordination and language development.la

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