This study investigated the use of Bacillus subtilis protease powder (CTC E-ssentials TM MT-70N) as a carabeef tenderizer. The effect of the bacterial protease on the characteristics of carabeef was determined, and its effectiveness was compared to a commercial meat tenderizer containing papain. Only B. subtilis protease showed significant enzyme activity (80-190 U/g), while the commercial meat tenderizer had no activity (0 U/g). Results from the shear force device revealed that 0.35% B. subtilis protease was the optimal concentration required to induce significant tenderization in carabeef (282 g/cm 2 ) and reduce carabeef toughness by 80%. Proximate analysis showed that carabeef treated with B. subtilis protease had significantly higher crude protein (37%) than the negative control (34%) and carabeef-treated commercial meat tenderizer (31%). Sensory evaluation revealed that carabeef treated with 0.35% B. subtilis protease is more tender than untreated carabeef and those treated with the commercial meat tenderizer. Moreover, the carabeef was not over-tenderized and is palatably acceptable. Hence, B. subtilis protease can be used as a meat tenderizer in place of available commercial tenderizers containing plant-derived proteases.

Aeromonas hydrophila is the most common opportunistic pathogen that plagues freshwater and euryhaline fishponds. Here, we present the complete genome sequence of A. hydrophila strain LP0103, which was isolated from a bacterial septicemia outbreak among suckermouth catfish ( Pterygoplichthys pardalis ) at Lotus Pond in Kaohsiung City, Taiwan.

Microbial colonization and the formation of biofilms on catheter surfaces pose a great risk for medical-related infections. We aimed (a) to evaluate polymicrobial biofilm formation of Candida albicans and Staphylococcus epidermidis and (b) to investigate the inhibition and effects of ethanol (EtOH) and EtOH–EDTA solutions on biofilms. Catheter disks were made and used as a substrate for biofilm formation. Varying concentrations of EtOH and EtOH–EDTA solutions were compared in deterring biofilm formation. The EtOH–EDTA solutions were further tested to remove mature and preformed biofilms. Compared to their monospecies counterparts, biofilm concentration significantly increases when C. albicans is co-cultured with S. epidermidis. Moreover, all treatments with EtOH–EDTA solution significantly lowered biofilm formation compared to EtOH alone (P ≤ 0.05). Lastly, biofilm was dramatically reduced when treated with 20%, 30%, 40%, and 50% EtOH–EDTA solutions (P ≤ 0.05). Our findings suggest that biofilms become more resilient to treatment when formed by multiple organisms. Nonetheless, treatment with EtOH–EDTA is effective against these polymicrobial biofilms.

Microbial-induced calcite precipitation (MICP) is an eco-friendly technique used in creating better soil substrate often for engineering purposes. This is done through the application of the ureolytic pathway of certain bacteria. This study aims to discover whether any of these bacteria can be found in Bukilat Cave, Camotes Islands, Cebu. Samples from the pools of water, drip water, and swabs of the walls of the cave were collected, cultured, and then tested using Christensen’s agar for their ability to undergo the ureolytic pathway. The rate at which they undergo the ureolytic pathway was then measured and compared between different sources and to the positive control, Bacillus megaterium. The results showed that there was no significant difference between the rate at which bacteria from the different sources underwent ureolysis. There was also no significant difference between the rate at which the collected bacteria underwent ureolysis and the rate of the positive control (2.588 mM/min). Finally, the species with the fastest rate of ureolysis was identified to be Bacillus cereus NR 074540 with a rate of 3.033 mM/min. However, it is not ideal for MICP purposes because of its potential pathogenicity.