The viable cell count performance of nutrient agar prepared by the MikroClaveTM sterilization system MSS‐500 was evaluated using Escherichia coli 0157:H7, Salmonella typhimurium, and Yersinia enterocolitica with conventionally autoclaved media as a comparison. No significant difference was found between agar prepared by the two methods. The MikroClaveTM was able to sterilize common bacteriological media such as Eosin Methylene Blue (EMB) agar, nutrient agar, Yeast and Mold (YM) agar, lactose broth, nutrient broth, and Yeast and Mold (YM) broth.