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SynopsisA method is presented which allows one to calculate a distribution of sedimentation coefficients from the boundaries of sedimentation-velocity experiments with mono-or paucidisperse solutes. With two solutes differences in S value as small as 20% can be resolved. In absence of heterogeneity and concentration-dependent effects, the analysis also provides values for the diffusion coefficient within an accuracy of -10 to +5%. Tests with both simulated data and ultracentrifugation experiments on short DNA fragments show the value and the limitations of the method.