In mouse atrium, M₂ and M₃ muscarinic receptors (M₂R and M₃R) are involved in biphasic (negative and positive) inotropic actions of muscarinic agonists, and the positive inotropic action is reduced by indomethacin. The aim of our study was to determine the localization of M₂R, M₃R and cyclo-oxygenase (COX) in mouse atrium and to characterize muscarinic receptor-mediated positive inotropy. M₂R immunoreactivity was found only on atrial myocardium, but M₃R immunoreactivity was localized on both the myocardium and endocardial endothelium. COX-1 and COX-2 immunoreactivities were identified in both myocardial and endocardial endothelium. In electrically stimulated left atria, carbachol caused M₂R-mediated negative inotropy followed by M₃R-mediated positive inotropy. Removal of atrial endothelium reduced the positive inotropy without affecting the negative inotropy, suggesting that stimulation of endothelial M₃R mediates the positive inotropy. N-[2-(cyclohexyloxy)-4-nitrophenyl]-methanesulfonamide (NS398, COX-2 inhibitor) decreased the carbachol-induced positive inotropy; however, 5-(4-chlorophenyl)-1-(4-methoxyphenyl)-3-trifluoromethylpyrazole (SC560, COX-1 inhibitor), 1-[[4,5-bis(4-methoxyphenyl)-2-thiazolyl]carbonyl]-4-methylpiperazine (FR122047, COX-1 inhibitor) and L-nitroarginine methylester did not affect the inotropic response. M₃R activation caused positive chronotropy in spontaneously beating right atria when M₂R-mediated negative chronotropy was suppressed and rate of contraction was low, <350 beats min⁻¹. Our results indicate that although M₃Rs are located on both myocardial cells and endocardial endothelial cells, only endothelial M₃Rs mediate positive inotropy in response to muscarinic agonists via activation of COX-2 in the mouse atrium. M₃R-mediated positive chronotropy counteracting M₂R-mediated negative chronotropy was also demonstrated.

Trueperella pyogenes is an opportunistic pathogen that causes a wide variety of purulent infections. We recently isolated a T. pyogenes strain unable to be identified by the previously reported T. pyogenes pyolysin gene (plo)-specific PCR from the lung of a sheep with astasia. Sequence comparison of plo among representative strains revealed several nucleotide substitutions in the primer-annealing regions. As such substitutions were considered to be a reason for the low PCR specificity, we designed novel primers in conserved regions of plo. Under optimized conditions, the novel primers precisely identified all T. pyogenes strains tested, and no products were generated from any other bacterial strains, suggesting the usefulness of the novel PCR assay for the diagnosis of T. pyogenes infections.

No abstract

&lt;p&gt;In this presentation we introduce coupled assimilation activities conducted in support of seamless Earth system approach developments for Numerical Weather Prediction and climate reanalysis at the European Centre for Medium-Range Weather Forecasts (ECMWF). For operational applications coupled assimilation requires to have reliable and timely access to observations in all the Earth system components and it relies on consistent acquisition and monitoring approaches across the components. We show recent and future infrastructure developments and implementations to support consistent observations acquisition and monitoring for land and ocean at ECMWF. We discuss challenges of surface sensitive observations assimilation and we show ongoing forward operator and coupling developments to enhance the exploitation of interface observations over land and ocean surfaces. We present plans to use new and future observation types from future observing systems such as the Copernicus Expansion missions.&lt;/p&gt;

False memory is a phenomenon of "remembering what has not happened" or "remembering what quite differently from the way they happened." In many studies with DRM paradigm which easily generates this phenomenon, the experimental participants perform the cognitive tasks alone. In this study, we analyzed an influence of relationships with others on the generation of false memories. Participants in this experiment memorized words selected based on the DRM paradigm and shared the information about the words with the agent with text-to-speech software through an online conferencing system. We analyzed the influence of explicit information on the reliability of the agent, the influence of the impression factor of others, and the influence of confirming the relationship through courtesy. As a result, the rate of false memory increased when the explicit reliability level matched the perceived reliability level of the participants, and the rate of false memory generation for male agents was higher than for female one. The results also indicated that the rate of false memory generation increased when the participants performed polite behavior. The lower rate of false memory generation than in previous studies suggests that interaction with the agent inhibits false memory generation.