The quality indices and chemical composition of ten common olive cultivars grown in different regions of Australia were evaluated to determine the diversity of olive oils produced in Australia. Olives from trees from different environments including warmer climates in the north to colder climates in the south were sampled at two different stages of maturity over 2 years. The oil was extracted and standard methods were used to analyse the oil. Oleic acid content ranged from 83.4% in the Picual cultivar grown in Tasmania to 54.5% in Arbequina grown in northern New South Wales/southern Queensland. The Barnea cultivar, which is very commonly grown in Australia, was above 4% for campesterol content, regardless of the region in which it was grown. Parameters, such as palmitic acid, oleic acid, linoleic acid and wax content were found to be significantly affected by growing region for some cultivars. This study shows the growing conditions for olive in Australia gives rise to a diverse range of olive oils.