We used community-based participatory research with four tiers of governance and grounded theory methods to examine the lives of Alaska Native grandparents rearing grandchildren in a rural community. We explored the reasons Native grandparents reared or are rearing their grandchildren, challenges rearing grandchildren, and the joys of grandparenthood. We used their words to broaden the grandparenting research by focusing solely on Native People living in a rural, arctic climate. Culture, values, and traditions explain the unique reasons grandparents cared for their grandchildren; values and common concerns of all grandparents describe the challenges of grandparenthood; pride and joy, the value of love, and traditional ways of living made clear the joys of Alaska Native grandparents rearing their grandchildren. After the discussion, we offer considerations for future research.

To investigate the cellular origins of cathepsin D (CD) in inflammatory lesions, the CD content of lymphocyte subsets, monocytes, and macrophages were compared. Human monocytes, B lymphocytes, CD4+ T lymphocytes, and CD8+ T lymphocytes were separated from peripheral blood of normal donors. CD content was 0.13 +/- .01 micrograms equivalents of CD per million cells and significant differences between different cell types were not found. To determine the CD content of macrophages, differentiation of peripheral blood monocytes was induced by either in vitro culture or treatment with 4 beta-phorbol-12-myristate-13-acetate (PMA). Macrophages induced by five-day culture contained four times more CD than unstimulated monocytes, and macrophages induced by 18-h treatment with 20 mg/ml 4 beta-PMA contained nine times more CD than monocytes treated with 4 alpha-PMA, an inactive stereoisomer of 4 beta-PMA. These results suggest that macrophages are one of the enriched sources of CD in inflammatory lesions.

No abstract

No abstract