No abstract

Improvements in conventional filtration and centrifugation procedures made it possible to increase the yield of intact cyto plasm-free pea (Pisum sativum) nuclei from the usual 3 to 10% to 32% (6 X 10' nuclei per 7 grams fresh weight of pea apices) and to complete the isolation in 80 to 90 minutes. The isolated nuclei appeared to retain their structural integrity as revealed in electron photomicrographs, and remained intact for at least 5 hour at 20 Cesius.The DNA:RNA:protein ratio of isolated pea nuclei was found to be 3.1:1:9.9. Their

No abstract

A biotransformation procedure has been developed to prepare intermediates for the synthesis of 3-hydroxyphenylacetylene (3-HPA), a high-value specialty chemical used in the manufacture of acetyleneterminated resins. Pseudomonas putida F39/D containing toluene dioxygenase was used to oxidize a series of substituted benzenes to their respective cis-2,3-dihydroxydihydrobenzene(cis-dihydrodiol) derivatives which in turn could be converted to 3-HPA via a metaphenol intermediate obtained by acid-catalyzed dehydration. The eis-dihydrodiols derived from the oxidation of phenylacetylene, styrene, acetophenone, and bromobenzene dehydrated to yield predominantly ortho-phenols. Three different phenyl-l,3-dioxolanes were oxidized by P. putida F39/D to three previously undescribed cis-dihydrodiols. Unlike mono-substituted cis-dihydrodiols, phenyl-l,3-dioxolane cis-dihydrodiols were dehydrated to form meta-phenols exclusively. The dehydration products, 3-hydroxyacetophenone and 3-hydroxybenzaldehyde, are suitable precursors for the chemical synthesis of 3-HPA.