Changing trends in neonatal sepsis is documented worldwide. We reviewed blood culture reports over a 3-year period. Out of 1,020 blood cultures sent from 620 neonates with suspected sepsis, 127 (12.4%) were positive. Gram-negative and gram-positive pathogens constituted 68.2% and 31.78%, respectively. Klebsiella was the most common organism (37/127) followed by Staphylococcus aureus (25/127), Acinetobacter (18/127), Escherichia coli (18/127), and Enterococci spp. (12/127). Gram-negative organisms were highly resistant to ampicillin (84.8%), gentamicin (81.3%), and cefotaxime (71.3%). Carbapenem resistance was observed in 26.6% of gram-negative pathogens. Vancomycin resistance among enterococci was 40%, and 12% of S. aureus strains were methicillin resistant. Mortality rate was 29.13% and was highest (44.44%) in Acinetobacter sepsis.