Esse estudo analisou a condição reprodutiva, por meio da histomorfometria, de P. discolor coletados em fragmentos de Mata Atlântica do litoral sul de Pernambuco, durante as estações seca e chuvosa. Os animais são de coleção e foram classificados de acordo com a posição testicular (descendentes e não descendentes). Para as análises histomorfométricas, foram selecionados aleatoriamente 18 espécimes durante as estações seca e chuvosa, dos quais (n = 11) com testículos descendentes e (n = 7) com testículos não descendentes. Os resultados demonstraram que as maiores médias da área de ocupação dos túbulos seminíferos foram na estação chuvosa, independente dos espécimes apresentarem os testículos descendentes ou não. Isso pode estar relacionado a um maior investimento em produção espermática, já que na estação chuvosa, existe uma maior disponibilidade de alimentos devido às precipitações pluviométricas.
The aim of this study was to evaluate the histology and morphometry of endochondral ossification in limbs of rats submitted to usnic acid from Cladonia substellata (AHTI) encapsulated in PLGAmicrospheres. It was used 12 pregnant Wistar rats that were randomly distributed in the control groups (n = 6), which received 1.0 mL of saline solution; and treated (n = 6), that received 25 mg/kg/day of encapsulated usnic acid in PLGA microspheres by oral administration. It was analyzed six fetuses from each rat under study. At the 20th day of gestation were euthanized the females and their fetuses removed for histomorphometric analysis of the anterior and posterior limbs. The results showed the epiphyseal discs of the animals with cell alterations, as in the morphology and numbers of chondrocytes. It also showed a decrease in the amount of collagen type I collagen in bone tissue. However, this study showed low toxicity of the encapsulated usnic acid on bone development, when compared to its conventional dosage form. In this way, the toxicity of usnic acid can be reduced by encapsulation in PLGA-microspheres.
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