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Out of 96specimens; 52 oral cavity swabs and 44 urine samples were collected from thalassemic patients in Erbil city. The results showed that 32 isolates of Gram positive bacteria were identified depending on VITEK 2 compact system. On the other hand, the results showed that 3 genera and 14species of Gram positive bacteria were isolated and among the isolates the predominant obtained bacteria was streptococci which 13(40.625%) isolates belong to 6 species, followed by Staphylococci which 10 (31.25%) isolates belong to 5 species and 9 (9.375%) isolates of kocuria belonged to 3 species. Some virulence factors such as oxidase, catalase, coagulase, DNase, protease, lecithinase, lipase, gelatinase, urease, hemolysin and β-lactamase were studied and the results showed that the isolated bacteria were differ in their ability to produce these above mentioned enzymes. All isolated bacteria were tested for their ability for biofilm formation using microtiter plate assay and the results revealed that all isolates were biofilm producer. The susceptibility of bacterial isolates against 10 antibiotics was tested and it was found that the most of the isolated species were highly resistant methicillin with percentage 71.19% , followed by cloxacillin and tetracycline with percentage 52.74% and 52.5% respectively, and also it was showed variable resistance to other antibiotics. Another part of the study concerned with using polymerase chain reaction technique for detection of mecA gene .The results indicated that all isolated Staphylococci speceis were harboring mecA gene with the product size 533 bp except S. vitulinus.. Also was found mecA gene in one isolate of K.rosea with same product size of above mentioned mecA, whereas no genes were obtained from other isolated bacteria and the detected gene of mecA in S.aureus and K.rosea was sequenced to confirm the results of mecA gene.

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