Biological control by various natural enemies, including microbial organisms, is a potential alternative to chemical control in integrated pest management (IPM) programs (Pedigo, 2002). Due to their selectivity and safety, microbial biocontrol agents are considered economical and eco-friendly components of IPM programs. These agents are often compatible with other natural enemies (Lacey & Shapiro-Ilan, 2008). The most important group of microbial agents are entomopathogenic fungi (EPF) (Liu et al., 2017). Approximately 60% of insect pathogens are EPF (Liu et al., 2017). The mycelia of EPF can penetrate the insect integument directly, whereas other entomopathogens, including bacteria, viruses, and protozoa, need to be ingested to infect insects (Lovett & Leger, 2017). Thus, EPF can play an important role in controlling sap-feeding insects, such as hemipterans, in microbiological control programs (Broumandnia et al., 2021). Aphids are important sap-feeding pests in many crops (Blackman & Eastop, 2008). They can cause severe damage

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Entomopathogenic fungi (EPF) are the main microbiological control agents of insect pests. One of the key factors in the pathogenicity of EPF is the production of insecticidal bioactive compounds. Therefore, the metabolites of two isolates of EPF, Cladosporium cladosporioides (Fresen.) G.A. de Vries (AF98) and Acremonium zeylanicum (Petch) W. Gams & H.C. Evans (IR87), were analyzed. The presence of insecticidal toxic cyclic peptides such as Bassianolide in C. cladosporioides AF98 metabolites was detected by HPLC. Moreover, GC-MS analysis showed some toxic compounds, including 3,4-Dihydro-7,12-Dihydroxy-7,12-Imethylbenz[A]Anthracene, 1,2,3,4-Tetrahydro-1,1,4,4,6-Pentamethyl-5,7-Dinitronaphthalene, and 1,2,3,4-Tetrahydro-1,1,4,4,6-Pentamethyl-5,7-Dinitronaphthalene in the metabolites purified from A. zeylanicum IR87. Also, a few hazardous compounds, including 3,5-di-tert-butyl-4-trimethylsiloxytoluene, bis(2-Ethylhexyl) phthalate, di-n-octyl phthalate, 1-pentadecene, and 1-eicosene were found which might be toxic against insects. These results showed that multiple compounds are likely contributed to the insecticidal effects of the EPF.