Synthesis of nanoparticles by green methods with antibacterial properties is of great researchers' concern in the explored of new pharmaceutical and biomedical products. In this study, we synthesized a new product of nanosized particles of silver, non-toxic economy, clean, and conservator for energy. An environmentally friendly route is used for synthesizing silver nanoparticles (AgNPs) using an orange peel extract as both reducing and stabilizing agent at room temperature. The synthesized NPs were characterized using ultraviolet (UV)-Vis spectrophotometer, Zitasizer which measures the average size of the particles at about 91 nm, Fourier transform infrared (FT-IR) spectroscopy, scanning electron microscopy (SEM) equipped with the energy dispersive spectroscopy (EDS) and characterization using Transmission electron microscopy (TEM). The results confirmed that the orange peel extract is a very good bioreductant for the synthesis of Ag NPs and we investigated the synthesized nanoparticles as an antibacterial which showed that the biogenic synthesized AgNPs exhibit inhibition, and had significant antibacterial against both gram-positive and gram-negative bacterial strains.

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Purpose: To synthesize and characterize eco-friendly gold nanorods (Au-NRs) and to assess their effects against two bacterial strains. Methods: Synthesis of eco-friendly gold nanorods was done from an aqueous solution of chloroauric acid and cetyltrimethylammonium bromide by mixing Olea europaea fruit and Acacia nilotica husk extracts with the latter as a reducing agent. The synthesis was monitored by ultraviolet-visible (UV) spectrophotmetry and a zetasizer, while the morphology of the resulting nanorods was assessed by transmission electron microscopy (TEM) and scanning electron microscopy (SEM) coupled with an energy-dispersive spectrophotometer (EDS). The effect of the prepared eco-nanorods on growth of Escherichia coli and Staphylococcus aureus bacteria were investigated by agar diffusion method. Results: The synthesized Au-NRs were mostly rod-shaped, with mean size of 96 nm. They exhibited a potent antibacterial activity against Gram-positive and Gram-negative microorganisms (Escherichia coli and Staphylococcus aureus). Conclusion:The findings suggest that the nanoformulation of the biomaterial mix of Olea europaea fruit and Acacia nilotica husk extracts is a cost-effective, eco-friendly, and non-toxic synthesis of AuNRs which has therpeautic potential.

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