A total of 140 samples were collected from patients staying at Tikrit Teaching Hospital for three days or more in order to identify the most common bacterial isolates within the infections acquired from Tikrit Teaching Hospital and its relation to the age, gender and educational level of the patient. The samples included 75 urine samples, 28 stool samples, 28 wound swabs, and 9 sputum samples. The percentage of nosocomial infections was (45.7%), where the highest percentage were among UTIs (50.7%) from the total of (75) samples, followed by wound infections (42.9%) from the total of (28) samples, gastrointestinal infections (39.3%) from the total of (28) samples, and pneumonic infections (33.3%) from the total of (9) samples. It was noticed that hospital infections were more common in females, age group (51-89) years followed by the age group (5 years and less), and the uneducated (illiterate) where the number of hospital infected patients was 47 uneducated (illiterate) patients with 71.2%, while the number of educated patients was 19 patients with 28.8%. Various bacterial species were isolated in this study, including Citrobacter.koseri, E.coli, Staphylococcus schleiferi, Staphylococcus epidermidis, Proteus vulgaris, Staphylococcus saprophyticus, Klebsiella ozaenae, Klebsiella ornithindytica, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis, Prov. rettgeri, Staph. flexneri, Prov.alcalifaciens and K.pneumoniae.    http://dx.doi.org/10.25130/tjps.24.2019.006

A total of 140 samples were collected from patients staying at Tikrit Teaching Hospital for three days or more in order to identify the most common bacterial isolates within the infections acquired from Tikrit Teaching Hospital and its relation to the age, gender and educational level of the patient. The samples included 75 urine samples, 28 stool samples, 28 wound swabs, and 9 sputum samples. The percentage of nosocomial infections was (45.7%), where the highest percentage were among UTIs (50.7%) from the total of (75) samples, followed by wound infections (42.9%) from the total of (28) samples, gastrointestinal infections (39.3%) from the total of (28) samples, and pneumonic infections (33.3%) from the total of (9) samples. It was noticed that hospital infections were more common in females, age group (51-89) years followed by the age group (5 years and less), and the uneducated (illiterate) where the number of hospital infected patients was 47 uneducated (illiterate) patients with 71.2%, while the number of educated patients was 19 patients with 28.8%. Various bacterial species were isolated in this study, including Citrobacter.koseri, E.coli, Staphylococcus schleiferi, Staphylococcus epidermidis, Proteus vulgaris, Staphylococcus saprophyticus, Klebsiella ozaenae, Klebsiella ornithindytica, Enterobacter aerogenes, Providencia stuartii, Staphylococcus warneri, Staphylococcus aureus, Proteus mirabilis, Prov. rettgeri, Staph. flexneri, Prov.alcalifaciens and K.pneumoniae

The present study carried out in Azady teaching general hospital in Kirkuk city during the period from August 2017 to May 2018, to determine the frequency of ToxA gene and ExoS gene in P. aeruginosa isolates. Pseudomonas aeruginosa (100) isolates had been obtained from (1260) different clinical specimens, High prevalence of P. aeruginosa found in isolates obtained from burn swabs. Regarding to genders and ages, P. aeruginosa presented in females (58%) more than males (42%), regarding to age groups, P. aeruginosa presented more frequently (26%) in age group (20-30) followed by children (1-10) years that formed (22%). Molecular detection of virulence genes had been done by using conventional PCR technique, regarding to prevalence of ToxA and ExoS genes, ToxA gene presented (96%), ExoS gene (92%). Regarding to the distribution of genes in clinical specimens, ToxA gene present (100%) in all clinical specimens except in urine samples (90.9%) and low percentage found in sputum (50%). ExoS gene present (100%) in all isolates, while ExoS gene less frequently presented in ear swab isolates ( 83.3%) than other specimens, followed by urine specimens (81.8%), in bronchial washes isolates ExoS gene was absent. This study aims to determine the frequency of ToxA gene and ExoS gene in P. aeruginosa isolates that obtained from different clinical specimens.