The impacts of smoking on the biological functions of human body was studied in district Multan's habitants. In this study, the impacts of cigarette smoking on total blood count, lipid profile, and liver functioning were investigated, and in this regard, blood samples of selected people having age limits above and below 30 years, including both male & female, were taken. Blood samples of selected population were analyzed with the help of Nishter Hospital's Physiological Laboratory, Multan. The results were expressed as percentages and Mean ± S.E.M (standard error mean). Results showed that cigarette smoking has significant harmful impacts on the smoker's hematological parameters, lipid profile and liver functioning. It was also seen that smoking increased the level of cholesterol, triglycerides, and low-density lipoprotein (LDL) cholesterol in both male and female smokers. At the same time, results about liver functions showed that smoking decreased the level of bilirubin in both male and female smokers as compared to that of nonsmokers and also results showed that concentrations of aspartate transaminase (AST), alanine transaminase (ALT), and alkaline phosphatase (ALP) in both male and female smokers were increased as compared to that of nonsmokers. The concentration of hemoglobin, mean corpuscular hemoglobin concentration (MCHC), mean corpuscular hemoglobin (MCH), lymphocytes and platelets in male and female smokers decreased. Overall, this study showed that smoking can have negative impacts on the biological functions of the human body with any age limit.